| Cell as the biotic elementary structure and functional unit, its basic vital process such as energy conversion,information discrimination and transmit,material transport is closely correlated to biomembrane. And biomembrane is a supramolecule system, which is principally made up of lipids,proteins and sugars. Membrane protein is the main represent of biomembrane function, which also can be divided to peripheral and internal membrane protein, the latter is account to 70～80% of total membrane protein. Some membrane proteins insert intramembrane entirely or partly, but others distribute by transmembrane. Ion channel , a transmembrane protein, which can select proper electric charges to pass the hydrophilia porous channel, has significant regulated effects on cell physiological functions such as cell electrical signal creation and transmission,cell volume sustain,emiocytosis,reabsorption and so on. Membrane lipid is another necessary moity to cell membrane, generally, considered to have the ability to maintain membrane structure, but more and more researches show that there are lots of interactions between membrane lipids and proteins; furthermore, membrane lipids regulate many types of protein functions.Cholesterol is ubiquitous in cell membranes and its content changes during development, aging and in various diseases, suggesting that it plays an important role in modulating cell functions. Therefore, This study investigated whether changes in cholesterol content could modulate activity of the voltage-dependent K+ (Kv) channel and its main compositions: delayed rectifier potassium current (IK) and transient outward potassium current ( IA) in rat hippocampal neurons by using whole cell patch clamp techniques. Acutely depletion of membrane cholesterol by exposing the cells to...
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