Prokaryotic Expression Of Curcin2 Gene In Jatropha Curcas

Ribosome-inactivating protein (RIP) is a group of toxic proteins inhibiting prokaryotic and eukaryotic ribosome by a specific modification of the large rRNA. It exists popularly in high plants with functions of anti-tumour, anti-virus and anti-fungus.Jatropha curcas, belonging to Euphorbiaceae and thriving in many parts of the tropics and sub-tropics around the world, is a multipurpose plant with many attributes and considerable potentials. Curcin, a ribosome-inactivating protein, was purified from the seeds of Jatropha curcas . It's gene has been cloned and expressed in E. coil. Curcin2 is a induced protein of Jatropha curcas under stress conditions such as low temperature, drought and fungi, and belongs to ribosome inactivating protein. In order to study the structure and function of curcin2 Gene and lay the foundation of exploiting curcin2, two prokaryotic expression vectors of Curcin2 Gene were constructed. And the difference between this two prokaryotic expression systems of curcin2 was compared.The form of expressing curcin2 in transformant was studied further..A gene fragment encoding mature peptide of curcin2 was obtained by PCR...