| Salicornia europaea L., belonging to the genus Salicornia, Chenopodiaceae, is one of the most salt tolerant halophytes reported so far. Compared with S. bigelovvi Torr., it has a much wider distribution. Because of the high oil content in its seeds, it has a great potential to become an oilseed crop. Besides, it can also be used as a vegetable and feed. The tissue culture of S. europaea provides a useful tool for future transformation research and the functional analysis of stress relative genes. In present study, we developed an efficient protocol for plant regeneration of S. europaea via shoot organogenesis from callus cultures derived from mature embryos. With intact mature seeds as initial materials, callus was induced at hypocotyls after 3 weeks of incubation on MS medium containing TDZ 0.1 mg/L and NAA 1.0 mg/L. After one subculture, the callus differentiated into shoots at a frequency of 26.7%. An improving method with 2, 4-D short treatment was tested. Through which shoots in cluster were obtained after 6 to 8 weeks of culture and the increased differential frequency was 55.2%. The shoots were rooted when cultured on half strength MS medium with IBA, KN and activated charcoal within three weeks. Meanwhile the regeneration of S. europaea via direct adventitious buds was tested but failed.In addition, it was also tested to transform S. europae by the method of floral-dip used frequently in Arobidopsis.
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