Effects Of Olfactory Bulb Destruction On Behavior And Neurotransmitters Of KM Mice

Objectives To discuss the relation of olfactory pathway and learning and memory, we established olfactory bulbectomy (OB) mouse and chemistry lesioned olfactory-bulb mouse two models, and studied on the change of behavior and neurotransmitter of two models, so as to reveal the relation of olfaction damage and nervous system disease of clinic.Methods Sixty 6-week KM mice were divided into six groups, five male and five female in each group. Then we established the following groups: sham-operated group, partial-OB group, total-OB group, saline group, low toluene group and high toluene group. The mice were detected the follow behavioral tests post operation: olfaction discrimination memory test, olfaction six- arms maze test, mouse locomotive activity, mouse pole board test, mouse water maze test and mouse striding pole motion test. After operation 14 days, each mouse was sacrificed and brains were removed. The slices of frontal cortex, basal forebrain, amygdale, entorhinal cortex hippocampus, piriform cortex and olfactory bulb were stained with Hematoxylin-eosin, observed Nissl bodies with Nissl's staining and detected the expression of CHAT,5-HT, GABA and Glu by immunohistochemistry.Result ( 1 ) The results of behavior : â‘  total-OB mice showed decreased performance record in olfaction discrimination memory test .olfaction six- arms maze test, mouse pole board test, mouse water maze test :and revealed increased mouse locomotive activity. â‘¡olfaction discrimination memory test and olfaction six- arms maze test in high toluene group were inhibited markedly, and increased mouse locomotive activity was seen in high toluene group. (2) The results of histological test in mouse brain:â‘  gliocyte proliferation was observed in basal forebrain and...