Cytochrome c (cyt.c) located in the biological membranes of living cell, and it is a part of the electron transport chain. Its redox action in vivo may be considered as a model and test system for bioelectrochemical redox. Its electrochemical properties have been studied extensively.In order to understand the direct electrochemical behavior of cytochrome c and develop its potential application, we have carried on our work in this thesis. The content consist of two parts. In part one, a simple approach was used to fabricate DNA modified glassy carbon electrode. Because DNA on modified electrode plays a key role in promoting the redox of cyt.c. The direct electron transfer of horse heart (cyt.c) on DNA-modified glassy carbon electrode was achieved. The DNA-modified glassy carbon electrode could be applied to detect cyt.c. In part two, the applications of cytochrome c were discussed. We choice DNA and L-cysteine as linker to fabricated cytochrome c/DNA modified glassy carbon electrode and cytochrome c/L-cysteine modified gold electrode,respectively. Cytochrome c can keep its electrochemical activity on these modified electrodes. In acetate buffer solution(pH 5.0), cytochrome c/DNA modified electrode gave an excellent electrocatalytic activity towards the reduction of nitric oxide, and the catalysis currents were proportional to the nitric oxide concentration in the range of 4.0×...
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