One-step Synthesis Of Micron-sized Functional Polymer Microspheres And Its Interactions With Bio-macromolecules

Micron-sized polymer microspheres, due to its advantages of high specific-surface area, monodispersity as well as facile preparation and functionalization, have been one of focuses in the recent years. The application of polymer microspheres can not only substitute, in part, natural biomaterial but also expand its applicable range largely, thereby presenting a wide prospective for various fields such as biomedicine, materials, information, food, chemical engineer and biological fields etc.In the present dissertation, a series of tentative works involving the synthesis of micron-sized polymer microspheres and their functionalization, the interaction between the synthesized support and bio-macromolecules as well as the modification of classic theories were presented. For the synthesis, one-stepwise dispersion-polymerization was employed. Two micron-sized monodispersity polymers with bioactivity surfacially was prepared based on the participated monomer which contains active aldehyde and sulfonic groups. With bovine serum albumin as target molecule and the synthesized microspheres as support, the interaction mechanism of support-biomacromolecule was studied. Related information indicated that the interaction mechanism is much more complicated in comparison to classic theoretical systems including idealized Langmuir model and practical Freundlich or Temkin theories. To interpret the practical process better, the classic theories were developed and which ultimately presents a more logical result. Further information also reveals that the adsorption and immobilization of biomacromolecule such as enzyme have significant effect on the activity, stability and conformation etc. The immobilization improves not only the performance of thermo-resistance and the depositing stability, but also increases the capability of anti-acids or bases, which thus pave the way for longtime and consecutive uses.