Study On Bioactivity Of Endophytic Fungi From Mangrove Plants And Secondary Metabolites Produced By Fungus A-1-2-3

The hot spot for searching novel drugs has been focused on the much more complex marine resources instead of the traditional terrene habitat in recent years. Mangrove plants as important new resources for potential pharmaceutical because of their ecosystems that straddled the land and the sea, from freshwater to seawater, often with distinct microorganism species, have gradually attracted more and more attention of natural products chemists.In this study, the population fluctuation in the endophytic fungi of Bruguiera gymnorrhiza and Kandelia candel, collected from Fugong in Fujian Province, were studied for one year. And the antitumor, antimicrobial, AChE and Trypsin inhibition activities of their metabolites were investigated.290 strains endophytic fungi were isolated from 2 kinds of mangroves. The results showed the number fastigium of endophytic fungi from Kandelia candel and Bruguiera gymnorrhiza were in April and October to December respectivel- y.Penicillium,Alternaria,Dothiorell and the nonsporulating groups were the dominant genuses of the endophytic fungi from Kandelia cande ,while the endophytic fungi from Bruguiera gymnorrhiza mainly belonged to Cephalosporium,Alternaria,Penicillium,and so on.The differences of the host played an important role in the attribution of the temporal variations of different endophytic fungi.In the investigation of antimicrobial activities, 40 strains (about 13.8% of the total isolates) which showed antimicrobial activities against one or more sensitive microbes (Bacillus subtilis, Escherichia coli, Staphlococcus aureus, Candida albicans, Aspergillus niger) were screened out by using the agar diffusion assay. Using the method of MTT (Methyl Thiazolyltetrazolium), the isolates were screening for antitumor strains in vitro. The results show that 17 strains (5.9% of the total isolates) can inhibit the growth of Raji and/or KB tumor cell lines. The active strains obtained mostly belonged to Penicillium, Alternaria, Paecilomyces and other 7 genuse- s.The endophytic fungi from 2 mangrove plants also displayed seasonal variations in...