| Objective: To evaluate the application of DNA fingerprinting map made by two synthesized and non-radioactive labeled oligonucleotide probes for genetic monitoring in inbred strain mice by comparing the stability, reproducibility and reliability of these two probes. To provide more information to expertize the DNA fingerprinting technology for improving the genetic monitoring method of inbred strain mice as well as building a standard DNA fingerprinting data base of mice used among laboratory rooms at different places.Methods: DNA fingerprint map of BALB/c,C57BL/6J,DBA/2 and C3H inbred strain mice were generated by two different biotin-labeled probes, (GGAT)4 and (GTG)5, using Southern blotting for genetic monitoring of these mice. The reproducibility and superiority of these two probes were compared through analyzing the number and siize of mean bands, the mean similarity coefficient (x), the meanallele frequency (q), the breeds specific bands (s) and their percentage in differentprobes (S/T), and the probability of an identical DNA fingerprinting (p) within and between breeds.Results: The DNA fingerprinting patterns of individuals from the same strain were identical, whereas individuals those from different strain were dissimilar obviously. The finger maps were completely different with different probes. There were 8-12 distinguishable bands on every DNA fingerprinting map of BALB/c,C57BL/6J,DBA/2 and C3H inbred strain mice made by two probes, much of them distributed above 2 kb and each map had polymorphism. The similarity coefficient within breeds was found to be 0.92-1.00, the probability of an identical DNA fingerprinting in them was above 3.1×10-1, which was significantly higher than that between breeds (x =0.22-0.39,P <1.07×10-4) . In addition, the percentages of breeds specific bands generated by probe (GTG)5 were a little bit higher than that generated by (GGAT)4.Conclusion: The DNA fingerprinting maps made by synthesized non-radioactive labeled oligonucleotide probes (GGAT)4 and (GTG)5 are highly sensitive and stable, because these probes can be gotten easily, the Southern blotting process is shorter and more convenient, the genetic distance between individuals and strains can also be detected, and the: non-radioactive labeling technique can avoid contamination of radioactive isotope. Furthermore, the reproducibility and stability of (GTG)5 are better than (GGAT)4. Meanwhile, when we make DNA fingerprinting with two different probes, more individual specific information can be detected and the results are more reliable. Hence, these two probes have a vast application prospects in genetic monitoring of inbred strain mice, and may be extended to the heredity quality monitoring of other animals as well as clinical and forensic medicine.
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