Studies On Molecular Interaction Between The Oxidation Products Of PPD And DNA

The studies on the interaction between DNA and small molecules are central to nucleic acid research. There is a remarkable application in the research field of genetics, novel drug design, pharmacological application, p-phenylenediamine (PPD) is the most widely encountered primary intermediate in oxidation hair dye formulations. In this study, we use atomic force microscopy, agarose gel electrophoresis, UV/Vis spectrum and MS to assay the interaction between the oxidation products of PPD and DNA. We conclude that the mechanism and binding form of this interaction from nanoscale to molecule level.1. In this study, we first employed the atomic force microscopy to determine the interaction between the oxidation products of PPD and DNA. DNA condensation was found, according the AFM topography. This phenomenon was never been reported in early researches. The results indicated there was electrostatic force between the oxidation products of PPD and DNA. It supplied evidence for other research from nanoscale, and was used for reference in the future.2. From agarose gel electrophoresis, time dependent DNA condensation was recorded in the mixture solution. This is similar to AFM topography.3. With UV/Vis spectrum, we found hypochromic effect at 415 nm and red shift at 530 nm. It was shown there was intercalation binding some small molecules to DNA strands.4. From MS analysis, we found Badrowski's base (BB, m/z=319.3), the trimer of PPD, were formed. BB aqueous solution induced DNA condensation from liquid chromatography and TLC separating results.We conclude that there is distinct molecular interaction between BB aqueous solution and DNA. Hair dye may be dangerous for human health. So, we hope that the drug therapy for hair disease and substitute of hair dye was designed, with pharmaceutical herbs.