Molecular Cloning And Genetic Transfer Of Plant Symbiosis Receptor-like Protein Kinase Gene

In ecosystems, plant-microorganisms symbiosis is a ubiqutous biologyphenomenon. Most of the land plants can form mycorrhizal with gungus. It plays a vital role to plant develop, and enlarge absorbed area of plant root; it can enhance plant assimilate phosphor and other nutrition, produce growth hormone, so that promote growth of plant root, improve plant abiotic stresses and capability of integrate resist in the severe environment, it can secrete several antibiosis substances, restrain pathogen develop, some mycorrhizal fungi can form mycoparasites with pathogen that kill or dissolve them. In recent years the research shows when fungi break into plant, plant may give birth to several signaling moleculars and genes expression, such as symbiosis receptor-like protein kinase(SYMRK). SYMRK is required for a symbiotic signal transduction pathway from the perception of microbial signal molecules to the rapid symbiosis-related gene activation.The study cloning LsSYMRK gene from Lathyrus sativus L. by molecular biology techque. Sequence analysis shows that LsSYMRK was 2775bp in full length, and encoding 925 amino acid. SYMRK is a member of the large family of Leucine-rich repeat(LRR) receptor-like kinase in plants,and it possesses an extracellular domain, a transmembrane domain, and a cytoplasmic protein kinase domain.Flanking by LsSYMRK gene under control of CaMV35S, Ubiqutin and root special promoter, construct different sense and antisense expression vectors, the choose marker using phosphinothricin gene. The LsSYMRK gene transfer into Nicotiana tabacum var. Winsconsin38, Arabidopsis thaliana and Agrostis stolonifera through plant genetic engineering techque. PCR and Northern blotting proved the LsSYMRK gene have been transferred into plants and can express in plant normally.In addition, we attempt to study SYMRK gene in Nonlegume. An endophytic fungi was isolated from Festuca arundinaca, The fungus belongs to Gibberella. by 18S rDNA identification. RNA of Festuca arundinaca root with infect the fungi was extracted, and acquired anout 600bp fragment by RT-PCR. The fragment has high comparability with other SYMRK by blasta. The study of fragment may set up a solid foundation for giving further insight on SYMRK gene clone and function analysis.