CUMS Accelerate The Expression Of Nogo-A And Nogo-receptor In Brain Tissue Of Mice

Nogo, as a neuron inhibitory proteins, enriched in myelin and oligodendrocytes. The interaction between the Nogo-66 loop and Nogo Receptor evidently transduces signals from oligodendrocytes to neurons for inhibiting axonal regeneration. Studies showed the different expression of Nogo in mice when condition changed. In our study, we observed the expression of Nogo-A and NgR Protein in the brain tissue of mice with chronic unpredictable mild stress (CUMS) and to discuss the significance. RT-PCR was used to detect the expression of Nogo-A and NgR mRNA. The immunohistochemistry method and the western Blot were used to detect the distribution and expression of Nogo-A and NgR Protein in the brain tissue of both CUMS groups and the control groups. One-way ANOVA and t test were utilized to analyze the data. From our Results, we known that the expression of Nogo-A and NgR mRNA was significantly increased in CUMS grouPs in the brain tissue in one week and reached the Peak value at three weeks compared with the control grouPs(P<0.05),and then gradually decreased. The expression of Nogo-A and NgR protein was significantly increased in CUMS groups in the brain tissue in one week and reached the peak value at three weeks compared with the control group(P<0.05),and then gradually decreased. The neurons density decreased while the Protein content increased in one week,until three week,the cell density decreased continually and the protein content also decreased. Couclusions: The expression change of Nogo-A and NgR mRNA ,Nogo-A and NgR protein increased at first, and then decreased gradually; The expression of NgR protein was earlier than the Nogo-A protein,and keep the high level longer than Nogo-A, but the change trends are identical. Above results suggest that the increasing of Nogo-A and NgR Protein may be correlated with the CUMS injury and may inhibit the neuron regeneration.