| The feasibility of embryonic stem cells to combine with silk fibroin biomaterials andtheir biocompatibility were unknown. To research the differentiation of mouse embryonicstem cells induced by silk fibroin films, here first we explored suitable treatmentconditions of feeder layer cells and established a set of steady system to culture mouseembryonic stem cells in vitro effectively. Then, the adhesion to silk fibroin films andundifferentiated growth of mouse embryonic stem ceils were investigated by observationwith microscope, MTT assay and colony efficiency analysis. The peculiarity, markerexpression, inheritance and differentiation of these mouse embryonic stem cells on silkfibroin films were also assessed by alkaline phosphatase detection, immunocytochemistry,karyotyping and in vitro differentiation. Results showed the feasibility of combination andgood biocompatibility between mouse embryonic stem cells and silk fibroin films. Theestablished system to culture mouse embryonic stem cells on silk films could support theirproliferation with pluripotency and normal karyotype. These cells spontaneouslydifferentiated into multi-differentiated cells on silk films, which grown out of the EBs. Andvarious kinds of cells derived from embryonic stem cells could adhere to silk films,especially myocardium-like cells could maintain their contraction on the silk fibroinmaterial. Taken together, these results suggest that silk fibroin materials are particularlysuitable biomaterials for embryonic stem cells combination. This study may provideimportant experimental information for tissue engineering, of which embryonic stem ceilsare starting cells and silk fibroin materials are scaffolds.
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