In Phosphorus Ore Heavy Metal Element Examination And Its Resistant Fungus Research

Take the phosphorus ore as the main material production feed level calcium phosphate calcium phosphorus easy passive absorption, is the commonly used calcium phosphorus feed. At present, about 800,000 tons phosphorus ore and the product of calcium phosphate are exported in our country, which expressed a growth trendence. In the export quarantine aspect, the overseas customer request to carry on the trace amount examinati on the heavy metal element and the fluorine, the chlorine plasma, but there have no the unification standard of the internation,the nation and the profession. In order to solve the difficult problems of the trace element determination which related to the phosphorus ore and its product in the entering and leaving country examination quarantine,in this research,we used the GFAAS (Graphite Furnace Atomic Absorption Spectrometry ) to determine the trace amount of lead, the cadmium, the chromium plasma and other ions in the mineral feed calcium phosphate (phosphorus ore);and used the HG-AFS (Hydride Generation -Atomic Fluorescence Spectrophotometry ) to determine the trace amount of the arsenic and mercury ion in the mineral feed calcium phosphate (phosphorus ore). now we have established the examination method of the trace amount of the lead, the cadmium, the chromium, the arsenic, the mercury plasma in the phosphorus ore, Which caused the limits separately reduced: Lead 1.84 ng/mL, cadmium 0.27 ng/mL, chromium 2.55 ng/mL, arsenic 0.18ng/mL, and mercury 0.06 ng/mL. The determination uses the substrate match way disposition standard curve: in the standard solution, joins the corresponding to the concentration of the calcium oxide and the ammonium hydrogen phosphate. Various elements determination relatively standard deviation in 1.98%-8.15%, returns-ratio between 90.5% -105.8%.The massive productions and the use of phosphate fertilizer and the calcium phosphate feed additive, will certainly create the phosphate rock toxic, harmful elements and compounds in the biosphere and the release of animals, plants, soil, Air and water, ecological environment and the pollution, then harm to human health.The valid control of spreading and pollution of heavy metals in the environment has hence become an urgent matter of the moment. In this study, through tameness and induction of living creature's tolerance from dirty mud, using microbial technology we have cultivated some anti-heavy-metal microbes able to resist the pollutions from poisonous chemical elements such as arsenic, chromium, cadmium, hydrargyrum, and plumbum, four of which are fungi and five are bacteria. The atomic absorption spectroscopy and atomic fluorescence spectrophotometer analyses show that, to the same chemical element, fungi has stronger resistance than bacteria by one time or so. While fungi have the general tolerance of 600 ug/mL to these poisonous elements, bacteria have about 300 ug/mL. Among these elements, microbe have stronger tolerance to As⁵⁺, and weaker tolerance to Hg²⁺. Fungi have better resistance to heavy metals than bacteria, and their hereditary traits are also steadier. Bacteria have unsteady heredity, may loss their resistance easily in their generation. These anti-heavy-metal microbes may be used in water and soil polluted by heavy metals.