Study On Signaling Mechanism Of Troy, An Axonal Regenerative Inhibition Relative Receptor

The re-establishment of damaged axon tracts appears to be severely limited or impossible in the adult central nervous system (CNS) of mammals. Failure of axon regeneration in the adult mammalian central nervous system (CNS) is closely due to inhibitory molecules associated with myelin, namely Myelin associated inhibitory factors, MAIFs. It was then found that p75NTR and LINGO-1 was involved in transducing MAIFs'inhibitory sigal. Recent study demonstrated that TROY can form a functional receptor complex with NgR and LINGO-1 to mediate cellular responses to myelin inhibitors and active RhoA. But there are not evidence suggest how Troy affect RhoA. The results of our research are as follows: the part one, By GST pull down assay and Two Hybrid Screening system to find the signaling conductor downstream of Troy. Separate the binding protein by 2-DIMENSIONAL electrophoresis, discriminate and analysis the protein spot.by mass spectrum. The part two: Screening for small molecules and Identification of Polygalasaponin G-type saponins, a natural herb molecules could increased neural axonal outgrowth and inactive RhoA.