It's studied that the number of isolated zeros of the Mel'nikov function for a one-parameter Hamiltonian system under polynomial perturbations in this paper. The per-turbed system is (?)= -2y + 3λy2 + (?)P(x, y), (?)= -x - x2 + (?)Q(x, y), whereλis a small parameter, (?) is a perturbed parameter, (?),k is a positive integer big enough, degP, degQ≤n, and n is a non-negative integer. The system is simplified to a Bogdonov-Takens system with one-parameter by using coordinate transformation, which is (?) = y - 3λy2 + (?)P*(x, y), (?)= -x + x2 + (?)Q*(x, y), where degP*, degQ*≤n. According to the first-order Mel'nikov function M1(h,λ) in terms of the Taylor expansion about the small parameter A, one upper bound of the number of isolated zeros of M1 (h,λ) is given by Petrov's theorem. When (?)m/(?)λM1(h,λ)|λ=0 (?)0(m is a non-negative integer), B(2, n)≤n + m-1. Further more, it's obtained that the number of the limit cycles of the perturbed system under quartic polynomial perturbations is no more than 7, and it is no more than 4 under cubic perturbations.
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