Synthesization And Study The Electrochemical Active-inactive Pair Molecular Beacons Based On Coordination Compounds

DNA from a specific electrochemical sensors is the DNA sequence to identify material and electrochemical components. Specific sequence of DNA plays a selective recognition of the role of specific target genes, according to identify material in the electrochemical hybrid electrochemical signals before and after the change of target gene can be measured in the number and whether there. This use of the double-stranded DNA complementary base pairs of DNA in principle to develop biological sensing technology, bio-analysis of workers by the high degree of attention. At the same time electrochemical detection method for its high sensitivity, lightweight cheap, easy to carry, less energy-consuming, and can be combined with modern microelectronics technology, easy to realize the advantages of miniaturization, the researchers have been widespread concern. DNA electrochemical sensors in disease control, environmental testing and drug screening, and other aspects of the study will have far-reaching significance and value.Molecular beacon is also called molecular beacons, it is the rise in recent years the use of nucleic acid hybridization of the DNA molecule for testing the new technology, is fast, convenient features. The establishment of DNA molecules to study the composition, content to provide a rapid, specific and convenient new method, in the field of genetic testing has broad prospects. The traditional elements beacons (Molecular Beacons, MB) is based on the principle of fluorescence resonance energy transfer design oligonucleotide, with the MB of in-depth study, MB has been designed into a variety of different forms and functions of the probe And successfully applied to monitor real-time PCR, a rapid analysis of gene mutation, DNA and RNA testing, DNA hybridization and the kinetics of DNA / protein interaction studies.This paper use electrochemical technology, combined with molecular beacons technology complex type of electrochemical activity - activity of non-conversion of beacons, their acts of thermodynamics and electrochemical study of the complex electrochemical active - inactive a conversion of Molecular Superscript both the molecular beacons retain their own high-sensitivity, high specificity and the introduction of the electrochemical detection of the advantages of convenient and reliable, has a high sensitivity and specificity, while a complex of electrochemical activity - activity of non-conversion Molecular beacons greatly increasing the electrochemical molecular beacons thermal stability, improve the electrochemical molecular beacons melting temperature linked to real-time PCR, and in solution directly to the identification of biological molecules ideas. This complex of electrochemical activity - activity of non-conversion molecular beacon for genetic diagnosis, and PCR analysis of real-time monitoring provided a good idea for the development of gene chips to provide an effective basis.This paper is divided into four chapters:Chapter 1 IntroductionMolecular beacons on the main structure, principle, the main factors, application and development prospects at the same time outlined the electrochemical analysis of the characteristics, classification and in the application of DNA tests on the electrochemical and molecular beacons with the latest developments and Prospect. Finally this paper described the purpose and significance of research and new in it.Chapter 2 Synthesization and characterization of the electrochemical active-inactive molecular beacons based on coordination compoundsThis chapter elaborate on the stability of high fever electrochemical activity -activity of non-molecular beacons design and synthesis: a sensitive electrochemical signals in response to Carmine acid coupling to the molecular beacons, as the electrochemical active - inactive signal molecule, using elements Beacon credit card issuers structure and sequence of its role and objectives after the configuration change, Carmine acid molecule in the state association from the state of reconciliation (OFF / ON) inter-changes, which is in its electrochemical active-inactive. The ability to build a new non-fixed electrochemical molecular beacons; added metal ions form complex, molecular beacons to increase thermal stability. At the same time using infrared and ultraviolet spectrum of Carmine acid metal complexes and Carmine acid-metal complex electrochemical molecular beacons were characterized. The results showed that the addition of metal ions effectively improve the electrochemical molecular beacons thermal stability. As metals, ligand can change, so the electrochemical molecular beacons structural design of a wide range of flexibility. This series of studies on the electrochemical active-inactive of conversion of non-development of molecular beacons is of great significance.Chapter III The thermodynamics study on the electrochemical active-inactive molecular beacons based on coordination compoundsThis chapter on the design of the complex electrochemical active-inactive of non-conversion of beacons carried out a detailed Thermodynamics Research, a complex calculation of electrochemical activity - activity of non-conversion of beacons thermodynamic parameters, at the same time, the composition of different complexes The electrochemical activity - activity of non-conversion of molecular beacons differences were studied; theoretically different metal ions complex consisting of electrochemical active-inactive of non-conversion of beacons on the impact of thermal stability, results showed that metal Complex of electrochemical active-inactive of non-conversion molecular beacon has a high thermal stability, and the different composition of the metal complex electrochemical molecular beacons thermal stability of different. Complex of electrochemical active a conversion of high-molecular beacons stability, the solution demands a low turbidity, experimental speed, low background signal, without washing, equipment simple and easy to operate, facilitate field testing and chip technology, Fluorescent molecules to overcome the lack of some beacons. Complex of electrochemical active-inactive of non-conversion of beacons will be directly in the hybrid solution to identify and realize the living cells to identify direct electrochemical detection, and real-time PCR detection of electrochemical applications.Chapter IV The electrochemical study on the electrochemical active-inactive molecular beacons based on coordination compoundsWill be electrochemical detection technology and high specificity of the molecular beacons technology is the combination of DNA analysis detected the trend, and how to electrochemical molecular beacons for solution testing, improvement of thermal stability, real-time PCR testing is electrochemical molecular beacons Key. This chapter of the heat-stable electrochemical activity - activity of non-conversion of a beacon of Electrochemical Research results show that the addition of metal ions to improve the electrochemical activity - activity of non-molecular beacons conversion of thermal stability, its chain of temperature Tm . At the same time the addition of metal ions there is no change in molecular beacons hybrid process. In addition, the metal ions on the molecular beacons of the loop rate cycle has some impact: the addition of metal ions makes electrochemical molecular beacons in the beginning stages of heating significantly reduced the impact of recycling.