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Establishment Of Nitraria Sibirica Pall. Tissue Culture System And Research On Its Salt Tolerance Mechanism

Posted on:2009-01-24Degree:MasterType:Thesis
Country:ChinaCandidate:C X WangFull Text:PDF
GTID:2120360245987093Subject:Botany
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Nitraria sibirica Pall.is a species of Zygophyllaceae in Nitraria genus,which is an important kind of tree species to control sand.Not only its fruits have higher economic value,but also N.sibirica Pall.plays an important role on local environment protection.In this study,a tissue culture system of N.sibirica Pall.was established by its stem.then we discussed the mechanism of plant salt resistance of N.sibirica Pall.' test-tube plantlet,hoping to offer some references for the tissue culture and the mechanism of salt resistance of the salt plant.The main research conclusions were showed as follow:1.It is the first time that a tissue culture system of N.sibirica Pall.was successfully established by its stem section.May middle is the best time when explants are collected.The optimal medium for inducing adventitious buds from stem explants was MS+6-BA 2.0mg·L-1+NAA 0.5 mg·L-1,the rate of germination was 91.11%;the optimal medium for subculture culture was MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1,the multiplication coefficients was 4.53;the optimal medium for rooting culture was 1/2MS+IBA 0.5 mg·L-1,the rate was 96.67%.2.The callus formation and plant regeneration system were established by the leaf of test-tube plantlet,and it is also the first time.Cultured one week in the dark then changed in the Photoperiod of 14 h/10 h.The optimal medium for callus formation was MS+2,4-D 2.0 mg·L-1,the formation rates was 100%,the relation growth amount was 468.31%after 25 days.3.NACl(0,25,50,100,200 mmol·L-1) was added in the optimal rooting culture medium,N.sibirica Pall.'s test-tube plantlet were grown in these mediums.With the increase of NaCl concentration,fresh weight,dried weight and root/shoot rates were firstly increased and then decreased.Under 50 mmol·L-1 NaCl,test-tube plantlets' fresh weight and dried weight were more higher than others.It showed that 50 mmol·L-1 NaCl was the best salt concentration for the growth of test-tube plantlet.4.With the increase of NaCl concentration,contents of soluble sugar,proline were increased.Meanwhile the contents of chlorophyll were firstly increase and then decreased,and have the maximum value under 50 mmol·L-1 NaCl.The content of MDA in the leaf were firstly decrease and then increased,and have the minimum value under 50 mmol·L-1 NaCl.These showed that 50 mmol·L-1 NaCl is the best salt concentration for test-tube plantlet growing.5.With the increase of NaCl concentration,the concentration of Na+ in shoot and root were increased,under low concentration(≤50 mmol·L-1) NaCl,rapidly increased,but under high concentration(≥100mmol·L-1) NaCl,increased slowly. Meanwhile concentration of K+ in shoot was decrease,but concentration of K+ in root was increased and concentration of Ca2+ in shoot and root were decreased and Na+/K+,Na+/Ca2+ in shoot and root were increased.These showed that low concentration NaCl,Na+ rapidly increased in shoot in order to adapting to salt stress, but high concentration NaCl,K+ increased in root to antagonize Na+,in order to avoid salt damage.In conclusion,in the experiment,a tissue culture system of N.sibirica Pall.was established.It indicated that 50 mmol·L-1 NaCl was the best salt concentration for the growth of test-tube plantlet growing,and discussed that under NaCl stress,the mechanism of salt tolerance the test-tube plantlet of N.sibirica Pall.used by accumulation of Na+,K+.
Keywords/Search Tags:Nitraria sibirica Pall., tissue culture, test-tube plantlet, NaCl, cation, osmotic adjustment
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