Functional And Bioinformatics Analysis Of MPK17 Gene In Arabidopsis Thaliana

Arabidopsis thaliana has good characteristic such as small genome, simple structure,small plant,short growth period,high propagating rate,and so on,and the whole genome sequencing has been completed in December 2000.So we make research for the function of MPK17 with Arabidopsis thaliana which is model plant,MPKs is a Ser/Thr-type protein kinase which is existed widely to each eukaryotes.MPKs is make up of MPK cascade signaling with other kinesis,accepting outside stimulate signaling,and changing signal over to cell to regulate directly the deliver of special gene,and then controlling cell biological reaction, such as proliferation of cells,differentiation,transform,apoptosis etc.At present,Arabidopsis thaliana known to have 20 MPK genes, according to the conservative TxY die body in the amino acid sequence comparison,divides into it TEY and the TDY two hypotypes,may also divide into a TEY kind of activating enzyme A,B,the C three groups, a TDY kind of activating enzyme alone lists as D group.At present what knows most studies are most is A,B,the C Asia race's member,like MPK3,MPK4,MPK6,MPK7 of Arabidopsis thaliana.The D Asia race members in the Arabidopsis thaliana have not seen the report,our laboratory predecessor separates MPK17 transposable elements insertion mutation strain MPK17 from Arabidopsis thaliana transposable elements mutant storehouse,it belongs to the TDY race.Therefore with the research,the revelation contains TDY this conservative structure MPK17 gene function as well as in the adaptation environment and in the plant hormone equisignal transduction function.First,we make use of nucleic acid sequencing of Arabidopsis thaliana MPK17 which is provided with NCBI,and some on line and native bioinformatics software to analysis Arabidopsis thaliana MPK17 sequence with bioinformatics,the main contents contain:the analysis of nucleic acid sequence open reading frame,the analysis of protein structure,the forecast of the protein secondary structures and the evolution relationship analysis,the first fruit is that it is located to 199 510 bp—203 125 bp of second chromosome of Arabidopsis thaliana, and expressing with the division organizations of buds and flowers, many organizations and cells of flowers,leaves,root etc,and the most controlling is in the gene of preliminary stage and last stage.Unifies the laboratory predecessor to work,they already initially appraised the MPK17 gene to environment factor,particularly plant growth material IAA and,2,4-D have the obvious stress response.This transposable elements carries the GUS reporter gene has the special expression in the root system and the young leaf organization.Based on this,I through the massive experiments in IAA,NAA, TIBA,and 2,4-D four kind with different concentrations to examine the difference of main rooting length from MPK17 mutant and the wild on Arabidopsis thaliana,between wild and the MPK17 mutant the main rooting length does not have the obvious difference through data processing analysis discovery identical auxin identical concentration, indicated that the MPK17 gene function flaw,adult plant with wild does not have the difference on the phenotype,this explained that Arabidopsis thaliana MPK17 gene function to have the possibility is the redundancy, has the mutual function with other homologous gene between the substitution possibility,to verify the function of MPK17,it is necessary further studies through the construction the sibship near gene's double mutant.According to sequential analysis of the second chapter indicated that MPK9 and the MPK17 sibship is closest,through PCR,the resistance screens and RT-PCR to screen the MPK17 and the MPK9 single mutant, then undergoes the hybridization,produces a successful F1 generation of hybrid.In recent years,a number of MPK have been isolated in the plant, and has made the considerable progress in its action mechanism research aspect.In the plant the MPK research mainly concentrates on the double seed leaf pattern plant(for example Arabidopsis thaliana,tobacco and alfalfa and so on),are very few about the corn MPK aspect's research. Therefore we discovered and In silico cloning corn's MPK17 gene using the biological information sciences method in the corn and carried on the preliminary analysis to its nucleic acid and the protein sequence, indicated that in the monocotyledon plant corn also has the MPK17 gene, to develops in the corn the MPK type and its function mechanism research,manual control its growth and strengthens the resistance then the gain in yield to have the important guiding sense,has provided the rich rationale for better research MPK17.