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Study On Physiological And Protein Expression Differences Among Two Morphologic Filaments Of Spirulina Platensis

Posted on:2009-03-24Degree:MasterType:Thesis
Country:ChinaCandidate:Z K ZhaoFull Text:PDF
GTID:2120360272489908Subject:Aquatic biology
Abstract/Summary:PDF Full Text Request
Spirulina is one of significant important microalgae which has high value of nutrition,possesses unique funtion of health protection,and produces multiplicate bioactive compounds.Generally speaking,the filament of Spirulina is regularly spiral form;however,its filaments can change from spiral to other abnormal morphologies, such as different degree of spiralization curved and even linear shapes,under intimidation of some exoteric alterative or bad environmental conditions,for example low temperature,low light intensity,ultraviolet radiation and so on.While morphology altered,the characteristics of filament also change accordingly in nutriology,physiology,biochemistry,genetics and breed aquatics.Rapid development of techniques of molecular biology and proteomics are helpful to explore the mechanism of Spirulina morphological alteration.Algal trichomes in two different forms,spiral(S) and linear(L),were extracted and purified from Spirulinal platensis FACBH Nos.869,882 and 1124,and research was conducted with regard to the morphological characteristics,growth rate, chlorophyll a content,photosynthesis,protein content,and SOD enzymatic activity of the two forms of algal trichomes.The results show that different forms of algal trichomes do not have a significant difference in growth rate but differ in physiology and biochemical composition to varying degrees:(1) different forms of algal trichomes contain different levels of chlorophyll a,with spiral algal trichomes containing more chlorophyll a than linear algal trichomes;consistent with this difference,spiral algal trichomes have faster photosynthesis than their linear counterparts;and(2) different forms of algal trichomes are different in total protein and SOD enzymatic activity as well.In Nos.869 and 1124 algal strains,the SOD enzymatic activity of linear algal trichomes is higher than that of spiral algal trichomes by 13.8%and 29.5%,respectively,while in No.882 algal strain,the SOD enzymatic activity of linear algal trichomes is higher than that of spiral algal trichomes by 14.7%.To examine the molecule regulating mechanism of the formation of spiral algal trichomes,we analyzed the total protein of the spiral and linear algal trichomes of three algal strains.Two-dimensional electrophoresis results show 17 differentially expressed proteins.Of these proteins,nine protein spots are downwardly adjusted in linaer algal trichomes,so are eight protein spots upwardly in linear algal trichomes.A mass spectrographic analysis and database search found that possible connections between 14 proteins and the formation of the spiral algae and 10 of these proteins were discussed.CpcI,which is related to the changes of expression and activity of photo-pigment protein;Cell-wall hydrolase/autolysin,UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl undecaprenol N-acetylglucosamine transferase,UDP-N-acetylhexosamine pyrophosphorylase,which are participators in anabolism and catabolism of cell-wall;ABC transporter protein,which can transfer compounds between membranes stimulatively or repressively and then regulates environment in vivo;Light-dependent protochlorophyllide oxido-reductase,which regulates anabolism of chlorophyll a;OmpR,adjusting nutrition transportion; transcriptional regulator of MarR family protein,which regulates transcription of protein;Glu/Leu/Phe/Val dehydrogenase and Nitrogen regulatory protein P-â…¡,acting as regulation of amino acid metabolism in bad environment.
Keywords/Search Tags:Spirulina platensis, Morphogenesis, Differential proteomics
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