Study On Low Density Lipoprotein-based Targeting Nano-carrier

The etiology of cancer is multifactorial. Understanding the specific expressing receptor of cancer and devising strategies for the targeted molecular probe /drug delivery are very useful for the early diagnosis and therapies of cancer.The purpose of this study is to establish a LDL-based multifunctional nanocarrier platform, which can be high loaded with optical molecular probes and targeting deliver to the cancer cells overexpressing folate receptor. We distill the LDL that we need from the plasma by density grad centrifugation. Then we reconstitute the LDL by intercalating DiI in the phospholipid monolayer and reconstituting the DiR-BOA into the lipid core, attaching the FA to the Lys residues in apoB-100, forming a nanoparticle targeting to FA receptor. We analyse and calculate the absorption and emission to get the ratio of element of the nanaoparticle by the spectrum. Electron microscopy was used to determine the morphology and the size of the aqueous dispersion of nanoparticles. Cellular localization and fluoresence intensity of the labeled LDL was monitored by confocal microscopy and flow cytometry. Therefore, the mul-tifunctional nanoparticle platform has the advantage of biocompatibility and payloading multiplicity of molecule probe. It provides an efficiency way of targeting tumor imaging in vivo.