Requirement Of The Cis-acting Elements For Arabidopsis RPL36B Gene Efficient Splicing In Saccharomyces Cerevisiae

Pre-mRNA splicing or the removal of introns from precursor messenger RNAs depends on the accurate recognition of intron sequences by the splicing machinery.Because of the different splicing machinary between Arabidopsis thaliana and Saccharomyces cerevisiae, RPL36B of Arabidopsis is incapable of correctly removing intervening sequences from transcripts in S. cerevisiae.To study the mechanism,we made mutation in 5' splice site and replace 3' ss and branchpoint to be similar to the structure of RPL36B intron in Saccharomyces cerevisiae.Then transform to defect S. cerevisiae that endogenous RPL36 deleted and have exogenous RPL36B in YCPlac33 and test the splicing efficiency of the Arabidopsis RPL36B intron to find out the important cis-acting elements. We found out that the site-directed mutation of 5'ss and yeast branchpoint TACTAAC inserted promoted Arabidopsis RPL36B efficient splicing in Saccharomyces cerevisiae. However, Arabidopsis RPL36B gene that first intron replaced by yeast RPL36B intron can not to be spliced efficiency in SC-His+5FOA media even reduce contrast to incubate in SC-His media.