The Molecular Regulative Functions Of AtMYB103 In Anther Development Of Arabidopsis

AtMYB103 encodes a R2R3-type of MYB transcription factor which regulates normal anther and pollen development.The knock-out muant of AtMYB103,ms188,showed defective tapetum development,altered callose dissolution and surviving microspores lacked exine,resulted in male sterile phenotype.Base on the previous works, investigation of AtMYB103 in this study improve comprehension of the mechanism of anther development.The subcellular localization showed that AtMYB103 protein is localized to the nucleus;we identified that the activation domain was localized in 62 residues at C-terminus of AtMYB103 by yeast two-hybrid,which compose the functional structure of transcription factor with R2R3 DNA binding domain at N-terminus.Base on the primary regulatory network of tapetum development, the quantitive RT-PCR was performed to confirm the results.We further constructed the downstream gene driven by upstream promoter to determine the relationship of these key regulator of tapetum。For screening the interacting proteins of AtMYB103 and up-/downstream genes,we constructed the yeast hybrid library of Arabidopsis inflorescences.Furthermore,the transgenic line of AtMYB103 and TAP-tag fusion protein was obtained,which provide easy access to find the direct target of AtMYB103 by CHIP.These works lay the foundation for further investigation of the molecular basis of AtMYB103 in anther development.