Physiological And Biochemical Changes Of Red-ear Slider Under Difficult Conditions And The Application Of The Research Findings

Turtles are an ancient animal of Reptilia, it is one of the few reptiles which can be retained down after a long evolution about billions of years. Their unusual longevity has been attracting the world's attention. Turtle can regulate its own energy metabolism, physiological and biochemical characteristics to adapt to adverse environmental, these physiological and biochemical changes may be closely related to the tortoise's longevity. Some of the enzymes related to energy metabolism of turtles may play an important role in the applications of sports medicine.The researches in the thesis are summarized as follows:(1) We determined the serum activities of LDH, CK and the levels of lactic acid in red-ear slider under the conditions closed to natural hibernation and explored the energy metabolism of red-ear slider in hibernation. During the 6-month research time, we found:First, the LDH activity was remainly at a high state, and had a significantly decrease during the last two months. Second, creatine kinase decline rapidly in comparison with the beginning of the trial which decreased to one-eighteen. Third, ATP enzyme maintained at a relatively stable level, there were no significant differences. Fourth, the levels of lactic acid were significantly (P<0.01)increased by about 15 times compared with the start of the trial. The experimental datas show that the anaerobic glycolysis is the mainly energy supply for the red-ear slider in hibernation.(2) Turtle is a famous longlife animal. It has a strong hypoxia tolerance. Based on the analysis of red-ear slider serum component.In order to study whether the red-ear slider serum can improve the hypoxic tolerance of other animals. We designed a treadmill model:we trained a group of Kunming mice and then injected red-ear slider serum into mice blood vessel from caudal vein. We force the mice run to exhaust on a treadmill with the speed of VO2MAX at last. The result indicated that high-intensity (VO2MAX) treadmill time of serum group mice significantly increased compared with saline group. After exhaust exercise, activities of muscle creatine kinase, lactate dehydrogenase of serum group significantly higher than saline group (P<0.01). The volum of lactic acid were significantly higher than the saline group (P<0.01). Mice body weight of serum group significantly increased than saline group (P<0.01), the levels of serum urea nitrogen were significantly lower than saline group (P<0.01).