Characterization Of Mouse Myosin X Gene Promoter

MyosinX (Myo X) is a kind of recently discovered myosin in vertebrates, which is approximately 240 kDa. In addition to full-length Myo X, brain expresses a shorter form of Myo X that lacks a myosin head domain. This'headless'Myo X is thus unable to function as a molecular motor, but is otherwise identical to full-length Myo X and, like it, contains three pleckstrin homology (PH) domains, a myosin-tail homology 4 (MyTH4) domain, and a band-4.1/ezrin/radixin/moesin (FERM) domain. These two proteins are the different transcripts of Myo X gene.To analyze the transcriptional control of Myo X gene, 2000bp of the 5'-upstream sequences of the mouse full-length Myo X cDNA and headless Myo X cDNA were analyzed by the Bio-informatics. To functionally characterise Myo X promoter activities, the 5'-upstream sequences were introduced in pGL-3 basic vector and promoter activity was tested in different cell lines. Luciferase reporter assays identified full-length Myo X exhibiting high promoter-like activity, but low of headless Myo X.Furthermore, serial deletions of the promoter sequences were introduced in pGL-3 basic vector and promoter activities of these 5'- and 3'-deleted constructions was tested in 293 cell line .