Effect Of Benzene,phenol And Nitrobenzene On Misgurnus Bipartitus Acute Toxicity And Antioxidant Enzymes Activity

In this paper, we dealt Misgurnus bipartitus with benzene, phenol, nitrobenzene respectively, to study the three benzene drugs on Misgurnus bipartitus the acute toxicity and the treatment of three antioxidant enzymes in the body and liver after the change. The results were as follows:1. LC₅₀ of benzene, phenol, nitrobenzene poisoning of Misgurnus bipartitus in different times.LC₅₀ of benzene, phenol, nitrobenzene poisoning of Misgurnus bipartitus during 24h is 153.420μl/L,52.480μl/L,90.420μl/L; during 48h is 141.213μl/L,44.757μl/L,82.310μl/L; and 96h is 129.927μl/L,41.197μl/L,79.697μl/L. The toxicity of phenol on Misgurnus bipartitus is largest, followed by nitrobenzene, benzene is the least.2. The effect of three types of antioxidant enzymes with three drugs on Misgurnus bipartitus.After poisoned by three benzene drugs, the antioxidant enzyme of Misgurnus bipartitus changed obviously. The results showed that after poisoned by benzene, phenol, nitrobenzene 24h, 48h and 96h , SOD activity increased at first, then a downward trend at various periods. With the time lengthen, solution concentration increasing, a number of uncleared generate free radicals caused irreversible injury on cell, so a slight decrease in SOD activity, compared with the control group, change significant (P<0.05). In generally, SOD, POD and CAT are antioxidant enzymes, such as the role will be jointly produced by the body to remove reactive oxygen species, in the article the changes in the activity of CAT and SOD activity were basically the same. the POD activity decreased afterwards increased, compared with the control group, change significant (P<0.05). However, as the solution concentration increasing, stress time lengthen, the three antioxidant enzyme activity declined. The activities of antioxidant enzymes were not significantly relative to the exposure doses of pollutants. The analysis of relevance indicated that three kinds of antioxidant enzymes activities correlated. It showed the positive correlation between SOD activity and CAT activity when Misgurnus bipartitus were pressed by three kinds of drugs, which meant SOD activity accelerated CAT activity. In additional, it showed the negative correlation between SOD activity and POD activity, which meant SOD activity restrained POD activity. When Misgurnus bipartitus were expose to phenol and nitrobenzene after 96h, CAT activity would activate POD activity.After poisoned by three benzene drugs, the antioxidant enzyme of Misgurnus bipartitus in liver has change. Results indicated that poisoned by three benzene drugs, SOD activity and CAT activity decreased with the solution concentration increasing, but POD activity was opposite. In lower solution concentration, SOD activity increased with the time lengthen, while poisoned by the higher concentration phenol and nitrobenzene, it decreased after 72h;with the exposure time lengthen, CAT activity and POD activity decreased then increased.