Biologicle Toxicity And Oxidative Stress Of Two Kinds Of Polycyclic Aromatic Hydrocarbons On Ulva Pertusa

Ulva pertusas were used for the study, by adding PAHs phenanthrene and pyrene into seawater, with different concentrations and different treatment time for exposure experiment on Ulva pertusa, including biological toxicity and oxidative stress of phenanthrene and pyrene on Ulva pertusa. The toxic effects of pollutants to provide two kinds of basic research data, and preliminary evaluation of the state of pollution.Biological toxicity exposure test processing 96h, with growth inhibition and chlorophyll content were observed, the results: 1. After 96h exposure treatment in phenanthrene, the growth of Ulva pertusa was not inhibited at 0.1mg/L of phenanthrene, chlorophyll content was higher than that in the control, and inhibition of the rest each treatment showed significant differences; 2. after 96h exposure treatment in pyrene, the results of growth inhibition in a dose-response relationship, and chlorophyll content of all treatments group were lower than the control group.The time of oxidative effect experiment was 6h and 72h, MDA and H₂O₂ content, SOD and CAT activity of antioxidant enzymes and Mnsod and CAT expression levels as indices. The experimental results were: 1. MDA and H₂O₂ contents of treated samples were increasing. 2. Exposured to phenanthrene after 6h, at 0.1mg/L, SOD and CAT activity were activated and at 24.3mg/L, both of which are very significant differences, but the critical concentration is different; exposured to phenanthrene after 72h, SOD and CAT activity and the concentration of the phenanthrene were in a dose-response relationship; 3. Exposured to pyrene after 6h, at 1.5-13.5μg/L, SOD activity increased slightly, and the activities of the remaining ones were decreased; in each treatment group were lower than the control group, at 121.5μg/L, CAT activity is significantly; exposured to pyrene after 72h, SOD activity and concentration were in a dose-response relationship, CAT activity in treated group than the control group, at 364.5μg/L CAT activity was significantly. 4. With RT-PCR test method, exposured to phenanthrene after 6h and 72h, at 0.1mg/L, Mnsod and CAT expression were starting up; exposured to pyrene after 6h, at 1.5μg/L, CAT and Mnsod both were starting up; processing time increased to 72h, Mnsod and CAT expression was reduced.