The Production Of Dihydroxyacetone By Microbial Conversion

More than 30 strains capable of producing Dihydroxyacetone from glycerol were isolated from fifty soil samples collected from different regions, then a comparison with all these strains concerning their converting capability of Dihydroxyacetone was made. Moreover, the paper has done much research on the literature of Dihydroxyacetone production based on delqh6, one of these above strains and the whole process of this paper is as follows: First, it summarized the characteristics and uses of Dihydroxyacetone, described glycerol transport mode together with its metabolic mechanism inside some acetic bacterium and reviewed the literature concerning both microbial fermentation and enzymatic production of Dihydroxyacetone. Then, the paper set up the quantitative and qualitative analytical method of Dihydroxyacetone in the presence of glycerol, revised the periodatic analytical process of glycerol, made a screening of Dihydroxyacetone? producing strains and moreover, it also compared all these strain isolated from soil samples by their ability of converting glycerol to 1,3? Dihydroxyacetone, among this experiments, the highest conversion rate from glycerol to Dihydroxyacetone is 16%. Then,using the strain delqh6 of the highest converting capability, the paper studied the literature on microbial fermentation production of Dihydroxyacetone and meanwhile, much analysis, on the formation of glycerol dehydrogenase together withits characteristics and on the literature for the enzymatic productionof Dihydroxyacetone, was done based on the strain de1qh6. The paperinvestigated the main influential parameters on the production ofDihydroxyacetone, by adjusting environmental conditions and by modifyingmush ingredients and it found a optica1 li terature condi tion forDihydroxyacetone production. Inside this paper, the highest conversionrate from glycerol to l, 3--Dihydroxyacetone is over 25%. At last, acomparison with the above two method of Dihydroxyacetone productionmentioned in this paper was made and furthermore, it estimated thepractica1 uses of enzymatic production in the industry.