Analysis And Determination Of Pharmaceuticals By Capillary Electrophoresis

This thesis consists of five chapters.Chapter one: Introduction of the basic theory of capillary electrophoresis, the application of CE and its future directions.Chapter two: A CZE method is developed to analyze the active component chlorogentic acid in Chinese herb Lonicera japonica Thump. The analysis was carried out under a buffer condition of 1 Ommol/L borate + I 9mmol/L phosphate + 1 0%(v/v) ethanol (p1-1=8.86). The applied voltage was 16kV and UV detector was set at 214nm, In this method, the treatment of the herb was simple and the quantitative analysis was accurate.Chapter three: A MEKC method determining the active components (andrographolide, neoandrographolide and deoxyandrographolide) in Chinese crude herb Andrographis paniculata was developed. The electrophoretic condition was 15 mM sodium dodecyl sulfate (SDS) +30 mM borate (pH 9.5). Under the optimized condition, three active components in the samples extracted by the mixture of water and ethanol (3:2, v/v) were quantitatively analyzed, and some preparations of Andrographis paniculata (Chuanxinlian table and Xiaoyan Lidan tablet) extracted by the same solvent were also determined. In order to validate the MEKC method, HPLC method was presented to compare with MEKC in respect of separation efficiency, sensitivity, linearity and repeatability.Chapter four: We developed a MEKC method for direct determination of the content of synthesized cxNitamin E, It was found that under the optimum separation condition (7mM borate +14mM phosphate +1 5mM sodium dodecyl sulfate (SDS) +10mM sodium cholate (NaCh) +8% acetonitrile; pH 92) with UV detection wavelength at 21 4nm, 16 kV constant voltage and 260C constant temperature, c&Vitamin E and its isomers can be baseline separated and x-Vitamin E was quantitatively analyzed. In addition, the sample recovery, the limit of detection and the repeatability of the method were investigated. Moreover, the influences of various parameters on the separation such as SDS concentration, NaCh concentration, pH of the buffer and acetonitrile percentage were also discussed.Chapter five: The advances in capillary electrochromatography including the packing method, its stationary phases, Gradient elution and the limitations of CEC.