| Objective: According to the unique curative effects of traditional Chinese herbs and civilian medical prescriptions in dealing surgical wounds, the project aims to develop an occlusive function dressing (OFD), which is bacteriostatic, hemostatic, analgesic and detumescent and obviously penetrative, coinciding with the characteristic of south war zone where is hot and humid environment (HHE) and modern battle surgery. The wound healing, complication, disabled rate and death rate were systematically observed in animal models using the selected OFD, which may provide some useful experimental data for the manufacture of nursing dressings specifically to south war zone.Methods: (1) Six experimental groups were set. To optimize all the factors to form an ideal prescription, hemostatic, analgesic, bacteriostatic pharmacy experiments of the selected traditional Chinese herbs were studied using factorial analysis statistics model. The biological evaluation of the selected dressing was analyzed through three groups of SD mice (each n=10). (2) The suitable membrane matrix components were defined. The best ratios of membrane matrix and herbs for the OFD were scanned by six different matrixes in seven different parameters level according to well-distributed regulation design. (3) Closed soft tissue injuries and cut injuries model in hindlimbs often pigs was established and two groups, OFD and control, were set. The pigs continued to be bred 22 days, exposing to Tdb(33.0?.5) 癈, Twb(32.3+ 0.15) C, rH(68+3)% for 8 hours every day. The vital signs, the amounts of bacteria, the changes of bata-endorphin of plasma (P-EP), the wet and dry weight of injuried tissue, the pathologic changes of wounds, the informations of wound healing were systematically analyzed.Result:(l) The hemostatic, analgesic, detumescent and bacteriostatic effects are significant different in all six power and extract groups comparedwith saline control (P<0.01). By comparing with each factor, The hemostatic and detumescent effects of the I-extract was significant lower and the bacteriostatic rate was higher than those of other groups. The minimum inhibitory concentration (MIC) in these six groups were from 0.00625 g/1 to 0.025 g/1. The mean analgesic time of the Ill-extract group was obviously delayed and the mean body-twisting frequency was lower compared with those in other groups (P<0.01), while the hemostatic effect did not showed any difference (P>0.05).(2) The best components for forming membrane matrix were polyvinyl alcohol, CMC-Na, gelatin, kaolin and glycerin. By multiple regression analysis, seven parameters of six different matrixes showed significant difference (F=13.271, P<0.01) and the regression equations is effective. The suitable components of the matrix Xi=5.0, X2=3.0 , X3 =10, X4=2.0, X5 =5.0, X6=0.02 were defined after many repeated experiments.(3) In HHE, the results of experiment and control groups were compared as followed: ?Before injuried, there is no difference in body temperature in HHE state (P>0.05), while there was significant difference since the first day to the 5th day after injuried in HHE state (PO.01). There were different in body temperature before injuried compared with those in HHE state or the period after HHE in the same group (PO.01). ?The bacterial quantity in the wounds were lower in experimental group at the 4th and 8th hour in HHE and 24 hours after injuried than those in control (P<0.01). (3) There was no difference in tissue water content before injuried compared with that in HHE state (P>0.05), while significant difference 24 hours since injuried (P<0.01). The muscle fiber slightly swelled and muscle grudge became wider in HHE state and 24 hours after injuried compared with those in control. (4) There was no significant difference in the level of plasma B-EP before injuried (P>0.05), while it showed significant difference at o.5h after injuried, in HHE state or after HHE between the two groups (P<0.01). In experimental group, there was significant difference before injuried thanthat... |
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