High Performance Capillary Electrophoresis Techniques And It's Application On Traditional Chinese Medicines (TCMS) Analysis

Traditional Chinese Medicines (TCMs) are widely used in China. Now the most important work is study new experimental means and methods for TCMs quality control.In this dissertation, High Performance Capillary Electrophoresis (HPCE) was focused as research object, developing several methods to separate and determine some TCMs such as AndrographispanicuJataand Salviamiltiorrhiza. Also this technique was studied for TCMs finger print analysis.This dissertation makes up of two primary parts: the analysis of TCMs by HPCE techniques and studying the finger print of TCMs by HPCE, TLC and NIRS. The first part include chapter 1(1), chapter2 and 3, and the second part include chapter 1(11) and chapter4.The introduction showed in chapter 1 is divided into two parts. In the first part, the development, principles, equipment, separation modes and applications in TCMs analysis of HPCE were outlined. In the second part, we reviewed some analytical methods in evaluating and controlling the quality of TCMs, discussed the significance and possibility of the finger print in TCMs quality control and described the techniques and criterions in establishing TCMs finger print.In the chapter 2, a new micellar electrokinetic capillary chromatography method (MECC) was developed for the detection of andrographolide and dehydroandrographolide in Andrographis paniculata (Chuanxinlian) in a single run. Analysis was performed on a polyimide-coated fused silica capillary of 42.2 cm effective length and 75 Mm i. d.. The detection wavelength was 240 nm. According to the experimental results, the optimum separation condition was selected as: 20 mmol/L phosphate (pH 6.8), 10 mmol/L SDS buffer with 5% methanol as buffer, 25KV and 25癈. Under this experimental condition, andrographolide and dehydroandrographolide could be separated effectively in less than eight minutes. The calibration curves of the two components were all linear over the range of 10~100mg/L (r>0.98). The detection limits were 11.30 ppm for andrographolide, 2.6 ppm for dehydroandrographolide. The R. S. D. of peak area and migration time for 6 runs was 2.4% and 0.6% for andrographolide, 2.3% and 0.3% for dehydroandrographolide. Three different parts of Andrographis paniculata samples and its medicinal preparations were analyzed by this method. The recoveries for the two components are 95%~110%.The work in chapter 3 is to introduce a capillary zone electrophoresis method (CZE) for separation three active ingredients (DSS, PAH, PA) in salviamiltiorrhiza (Danshen).The buffer solution employed was 20 mmol/L phosphate, 5 mmol/L borax, pH 7.0. The capillary used was 75 Mm in i.d. and 50 cm in length. UV detection wavelength was set at 280 nm. The peak areas of DSS, PAH and PA and their corresponding concentrations have good linear relationships from 50 ppm to 500 ppm (r>0. 999). This method has been successful applied to the analysis of real salvia sample and its medicinal preparations.A CZE method for TCMs finger print analysis was describes in chapter 4. The capillary used was 75 Mm in i. d. and 32.2 cm in effective length. The optimum separation condition was selected as: 30 mmol/L borax buffer of pH 9. 2, UV detection wavelength 254 nm, 20KV and 25癈. By using this method, seven batches of Glycyrrhiza uralensis (Gancao) samples were analyzed and the contents of glycyrrhizic acid in these samples were determined. The experiment results indicated that the method, which was established in this work, is suit for the TCMs finger print analysis.