Ampelopsis grossedentata is a kind of rare tea-like plants, which belongs to perennial liana. It is rich in nutrients and functional ingredients; its flavor is light sweet and refrigerant. It is called miraculous tea which is not spoiled in summer for a few days. A lot of reports suggested that its extraction has the effects of resisting blood platelet coagulation and thrombus formation, reducing blood lipids, antioxidation, and sterilization, etc. With the material of Ampelopsis grossedentata, this objective was to analyze its nutrients and effective components, to search the fit conditions for extraction, and to grope the optimum directions of tea beverage. The desirable processing for tea beverage production was brought around based on these results. The main results are as follow:Ampelopsis grossedentata contains abundance mineral elements in which Ca, Mg, Fe, Zn is rich and trace functional ingredient of Se which is health-care. And it also contains the 17 of natural amino acids and special amino acids such as y-aminobutyric acid, methionine, glutamic acid and aspartic acid, 9 of which are essential acids for body. The major functional compositions are ampelopsin, amberin, p-sitosterol, myricetin, and myricitin. Although the essential oil was poor in Ampelopsis grossedentata, the 25 ingredients in it were identified through GC/MS analysis in which the phytol was 25.66%.The single factor experiments showed the ratio of water and the sample(A), extracting temperature(B), extracting time(C), and the sample particles(D) and the orthogonal experiments (L9(34)) revealed the influences of four factors on total flavones and water extraction ratio in the order of A, B, D, and C. According to the practical processing, the excellent method for extraction was modified to A2B2C1D1, that is to say, water and the sample is 1:20, the extracting time is 20min, the extracting temperature is 90@, and the sample needn't to be grinded. The extracting times indicated that the Ampelopsis grossedentata was perdurable to dunk and the water extraction ratio was very high under the optimum extracting conditions. The total flavones and water extraction ratio attained to75.87% and 80.56% for the first time, respectively.Based on the orthogonal experiments and comprehensive evaluation, the perfect direction for production of tea beverage is A2B1C1D2 which is composed of the extraction 15%, sucrose 4%, citric 0.11%, and stabilizing agent 0.08% by the addition of a proper amount of black tea, green tea essence, galacto-oligosaccharide, and glucose. The products held the proper scent and flavor from Ampelopsis grossedentata, was well situated in its sourness and sweetness and cool and clear in taste feel, and had the favorable health protection function. The advanced technology and equipment of cold-aseptic was introduced to the processing, which could avoid the oxidation and volatilization of aroma substances at high temperature and to a remarkable degree maintain these perfume and functional ingredients. The product which held the inherent flavor was found to be preserved for 6 months at the room temperature without any antiseptic. It is bright and lucid. |