Investigation Of Chemiluminescence Detection Coupled With Capillary Electrophoresis And Chip Capillary Electrophoresis

In this thesis, an ultra-sensitive method was developed to detect metal ions and polyamines by coupling the highly efficient capillary electrophoresis (CE) with extremely sensitive Chemiluminecence (CL). The research results obtained are as follows:1. An ultra-sensitive chemiluminescence detector was built in home and successfully coupled with a commercial capillary electrophoresis instrument. A commercial software was used for the CE instrument control and data collection.2. Based on the luminol-hydrogen peroxide reaction catalyzed by metal ion, CE-CL was applied for quantitative determination of Co(II) in vitamin B₁₂, and the detection limit of 2.5 x10-12 mol/L (S/N=3) was obtained for cobalt. Our data demonstrated that CE-CL system was successfully used for quantitative analysis.3. We first present a highly sensitive and universal indirect CL detection for CE of cations using cobalt(II) ions as probe ion based on displacement of probe ion by migrating sample ion. It was demonstrated that this method was successfully applied to detect metal ions andpolyamines ions, Their detection limits were 0.3-1.2x10" mol/L (S/N=3), which was about 3 orders of magnitude better than indirect UV detection, and 2 orders better than indirect laser-induced fluorescent detection. The data showed that CE with indirect CL detection was a powerful and universal tool for analysis of inorganic and organic cations.4. An ultra-sensitive CL detection system for micro-chip electrophoresis (MCE) was established and micro-chips were fabricated by poly(dimethylsiloxane) and glass in the laboratory. Optimization of detection models and reaction conditions were systematically carried out and the detection limits of 5x 10-11 mol/L (S/N=3) was obtained for cobalt(II) ion, which was about 4 orders of magnitude better than thereference. The separation of metal ions was achieved by MCE with CL detection.