| Resonance Rayleigh Scattering(RRS) is a new technology developed in 1990s. For its remarkable characteristics of high sensitivity, simple operation and good sensitivity. This method brings more attention and interesting and has been studied and applied more and more in recent years. This thesis introduced Resonance Rayleigh Scattering theoretical basement, the present situation,application and development as analytical technique .Then, the spectral characteristics of following three systems incluing berberine(BB) with sodium tetraphenylboron (NaTPB),some basic dyes such as triphenylmethane dyes, phenothiazine dyes,rodimine flouence dyes with sodium tetraphenylboron (NaTPB) as well as S nanoparticles for the exemples have been studied .1Resonance Rayleigh Scattering Spectra of Berberine-Tetraphenylboron Association Nanoparticle and Its Analytical Application In pH=5.0 NaAc-HAc buffer solution, BB- and TPB+ combine to BB-TPB association complex. The association complexes aggregate to (BB-TPB)n association nanoparticles, because there are strong hydrophobic force and Van der Waals force. The association nanoparticle between BB+ and TPB- has been investigated by means of RRS and absorption spectral method and transmission electron microscope(TEM). It exhibits a resonance Rayleigh scattering peak at 470nm, and a maximum absorption peak at 368 nm and a resonance absorption peak at 440 nm. Study the relationship between resonance Rayleigh scattering(RRS) and the association nanoparticle forming. A new and sensitive RRS method has been proposed for the determination of 0.06 to 5.28μg/ml BB in real samples, with satisfactory results. The detection limit is achieved at 26 ng/ml. The TEM of (BBj Ag p-TPBj+p)h composite association nanoparticles was observed by means ofcomposite association nanoreaction both TPB- and BB+ or Ag+. The results identify that the formation of (BB-TPB)n association nanoparticles and interface of solid-liquid results in the enhanced resonance Rayleigh light-scattering. This new RRS method has high sensitivity, and has been applied to the determination of BB in real samples, with satisfactory results.2spectral properties studies on the system of sodium tetraphenylboron (NaTPB) and some basic cationic dyesIn weak acid media, sodium tetraphenylboron (NaTPB) reacts with some basic triphenylmethane dyes such as Ethyl Violet(EV), Crystal Violet(CV), Methyl Violet(MV),Brillant Green(BG), with some basic phenothiazine dyes such as Methylene Blue (MB),Toluidine Blue(TB) and some flouence dyes such as RDG,RDB to form hydrophobic ion-association complexes,and association nanoparticles that result in a significant enhancement of Resonance Rayleigh Scattering(RRS) and appearance of a new RRS spectrum have formed spontaneously. Their maxium Scattering wavelengths λmax appear at 530nm, 528nm , 520nm, 525nm , 410nm, 405nm,420nm and 400nm for EV, CV, MV, BG, MB, TB, RDG and RDB ,respectively. the RRS spectral characteristics have been investigated. By combining ultra violet visible spectrophotometry and flouence spectrum, the reaction mechanism has been discussed. The results show that the RRS spectral characteristics of the combination products of sodium tetraphenylboron (NaTPB) with these basic dyes are closely related to their absorption.there is a corresponding relationship between them.In the meantime,the mechanism explantion on fluorescene quenching and enhanced Resonance Rayleigh Scattering(RRS) of some flouence dyes was proposed.3 Effect of Dyes on Resonance Scattering Spectra of S Nanoparticle Using polyacrylamide (PAM) as stabilizer, stable S nanoparticle solutions have been obtained by means of replacing solvent procedure. There is a maximum resonance scattering peak at 470 nm, and no absorption peak in visible light region. The effect of dyes such as bromphenol blue(BPB), bromomethyl violet, crystal violet and brilliant green, and some organic solvents such as acetone, alcohol and methanol on resonance scattering spectra of S nanoparticle has been studied. The... |
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