Preparation, Characterization Of Inorganic Nanoparticles And Application Of Inorganic Nanoparticles As Probe In Bioanalysis

The study of nano-materials has attracted tremendous attentions because of their potential utilities in many fields. For example, quantum dots can be used for fluorescene, photonics, and electrochemical applications in analytical chemistry for their excellent optical properties.In this work, Metal-enriched metal sulfide nanoparticles (MS-M2+,M=Zn,Cu,Pb,Ni,Cd) have been prepared. We found ZnS-Zn2+ and CuS-Cu2+ nanoparticles are water-soluble and biocompatible. All the nanoparticles have been characterized by UV spectra,Fluorescence spectra and TEM images.In comparison with organic dye probes,these nanoparticles probes are brighter,more stable against photobleaching,and do not suffer from blinking. Under the optimum conditions,the response is linearly proportional to the concentration of gamma-globulin human. ZnS-Zn2+nanoparticles as a RLS probe: between 0.1 and 2.0 mg/L, the limit of detection is 0.0403mg/L, and the linear correlation coefficient is 0.9974; CuS-Cu2+ nanoparticles as a RLS probe: between 0.1 and 1.5 mg/L? and the limit of detection is 0.0646mg/L,and the linear correlation coefficient is 0.9938.We find the effect of other protein on this assay is weak, this assay has good selectivity.The recovery and relative standard deviation are very satisfactory. We have also synthesized ZnO-capped ZnS semiconductor nanoparticles,et al.