The Study Of Cadmium-Arsenic Interaction In Wheat

Cadmiumn-Arsenic interaction was systematically studied in the thesis with the plant material of wheat ( Triticum aestivum L. ).1. Inhibition of cadmium-arsenic interaction to the wheat growth Results showed that cadmium would inhibit the wheat growth, arsenic would also inhibit the wheat growth. The wheat growth was inhibited deeper under cadmium-arsenic interaction. Through Growth Inhibition Curve, we got half growth inhibition concentration (IC50) and common safety concentration.2. The effect of cadmium-arsenic interaction on the absorption of cadmium and arsenicResults showed that, in the wheat root, arsenic significantly inhibited the uptake of cadmium( P<0.01 ). Meanwhile, in the wheat root, the arsenic concentration significantly increased( P<0.01 ).3. The effect of cadmium-arsenic interaction on the wheat NPT(Non-Protein Thiols) concentration Results showed that, in the nutrient solution with cadmium, with the increasing of cadmium concentration, the concentration of NPT increased; in the nutrient solution with arsenic, with the increasing of arsenic concentration, the concentration of NPT increased. Comparing arsenic added group and arsenic free group, in the same concentration of cadmium, the addition of arsenic significantly increased wheat NPT concentration( P<0.01 ).4. The effect of cadmium-arsenic interaction on the wheat phytochelatin concentrationBasic theory of phytochelatin analysis was introduced. Then, by mBBr pre-column derivatization method, we finished the phytochelatin standards analysis.5. The design method of heavy metal interaction experiments Basing on half growth inhibition concentration ( IC50) and common safety concentration, a new method of heavy metal interaction experiment design method was proposed.