| Used the fruits of Z Schinifolium sieb. et zucc as materials, the numb-taste components were isolated and purified with a series modern technology such as modern isolation and purification technology and instrument analysis technology. Used the purification of numb-taste components as comparison, the quantities of numb-taste components from fruits of Zanthorylum bungemm, oil, and resin were inspected by HPLC, GC and ultraviolet spectrometer. The quantities of water, volatile oil, resin and numb-taste components of fruits with different picking-time were determined. The result indicated that:1. The technology of separating and purifying numb-tasted components:Zanthorylwn bungenum resin →separated with solvent →separated in dry silica gel column with contrary-direction methods → collected the second and third groups →separated in silica gel column eluted with different solvents →collected the peak groups detected by ultraviolet spectrometer →frozen in crystallize in petroleum ether →crystal solved by methyl alcohol →purifled by pre-HPLC →collected the peaks group detected by ultraviolet spectrometer →frozen in crystallize in petroleum ether →crystal (PCl,PC2, PC3)2.The crystals are white or yellow substance which have strong pungent, and the yield rate of PCl, PC2, PC3 reach 0.15%,0.19%, 0.09%. When the crystals are placed in a circumstance with normal temperature and atmosphere, they will turn into yellow viscous substance; when the temperature is 0℃ and nitrogen is filled, only a little of them turn into yellow viscous substance in 15mSnutes; when they are placed in petroleum ether, it is very stable.3. Identified crystal (PCl, PC2, PC3) by thin-layer chromatography, there are only one pot on the thin-layer and the Rf value is 0.35; Identified by HPLC, there are 3 peaks; identified by GC-MS, there are 8 peaks and the mass spectra of crystal are similar to unsaturated alkylamides'. The numb-taste materials of the fruits of Z Schinifolium sieb. et zucc are a mixture of 8 unsaturated alkylamides. The pureness of crystal PC2 is 81.04%, which is the highest of the three.3. Used crystal PC2 as comparison, the GC method was built up to decide the quantities of numb-taste components. The average rate of recovery is 87.8%-104.3%; the coefficient of variation of repeatability test is 1.57%; the limit of detection is 0.055 ug/mL.4. Used crystal PC2 as comparison, the HPLC method was built up to decide the quantities of numb-taste components. The average rate of recovery is 88.1%-104.3%; the coefficient of variation of repeatability test is 1.57%; the limit of detection is 0.044 u g/mL. This method has been used in Chong Qing Si-mian mountain prickly-ash exploit limited company for 7 months, and 76 batches of samples have been analyzed, and 652 data have got, which induced the method is reliable.5. Used crystal PC2 as comparison, the ultraviolet spectrometer method was built up to decide the quantities of numb-taste components. The average rate of recovery is 81.3%-110.2%, the efficient of variation of repeatability test is 3.71%, the limit of detection is 0.692 ug/mL.6. Used GC method, HPLC method and ultraviolet spectrometer method, the contents of numb-taste substances in pericarps of Zanthorylum bungenum are 15.39 mg/g, 15.15 mg/g, and 14.71mg/g, which in oil are 1.74mg/g, 1.68mg/g and 1.85mg/g , which in resin are 200.42mg/g , 196.65mg/g and 188.81 mg/g respectively. By test of significance ,the results are not significant when the significance level is 0.05 .
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