Elementary Study On The Fermentation Of Monascus And Its Trend Growth

Monacolin K is secondary metabolite of some Monascus, which is a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in cholesterol biosynthesis, thereby is effective in the therapy of hypercholesterolemia, besides, Monacolin K has recently been indicated as potential therapeutic agent for the treatment of various type of tumors.In the essay M.ruber was considered as tool of production, in order to improving the productivity of Monacolin K and researching the trend growth of microorganism which close with nutrition.1. Measure the content of Monacolin K by Uvspectrophotometer. From a series of experiments we draw a conclusion that use 70% ethanol as solution to extract Monacolin K 8hrs and 236nm is the maximal wavelength of Monacolin K in 70% ethanol solvent.2. Factor test indicated that glycerol and peptone benefited obviously the synthesis of monacolin K. Furthermore we studied the optimal concentration of glycerol and peptone.3. From co-culture and non-coculture of Mruber and Saccharomyces cerevisiae, we found that during the two culture condition Saccharomyces cerevisiae could increase cell mass and the yield of monacolin K, but the co-culture is better than the co-culture to improve the Monacolin K of M.ruber, the possible reason was during co-cultured the effect of Saccharomyces cerevisiae became direct.4. During the liquid, solid and surface fermentation of Monascus, respectively adding Yeast lysate, Yeast and Yeast filtrate, indicated that the best inducer in liquid, solid, and surface culture was Yeast lysate, Yeast and Yeast. Furthermore, when Monascus cultivate in liquid method, adding Yeast filtrate in the beginning of Monascus fermentation and the quantity is 2.7% (v/v), the yield of Monacolin K reaches 61.93mg/L. Study on variance of Monascus biomass show Yeast filtrate could improve the biomass of Monascus and metabolize path, so increase the yield of Monacolin K.5. In the test M.ruber was considered as tool of study, a series of experiment testified that atmosphere was essential condition of the trend growth of microorganism.6. we research that different nutrition effect the trend growth of microoganism, the result was the best nutrition is malt extraction, secondly glucose, the worstis water. Three experiments testified that nutrition was also essential condition of the trend growth of microorganism.