| Korean pine (Pinus koraiensis Sieb.et Zucc.) can provide us with not only valuable timber, but also famous, high-quality and special dry fruit. Its pine nut contains a large amount of biological active composition and rich nutrition.1.The unsaturated fatty acid in Korean pine nut was extracted by the method of supercritical CO2. Regard extraction pressure, extraction temperature and extraction time as the factor, carry on the experiment of the factors that impacts on the yield respectively, on the basis of this, have settled three factors and three levels by orthogonal designs. Determine the best technology parameter, namely the extraction pressure as 40 MPa, extraction temperature as 50C, extraction time as 3h. Carry on Korean pine nut unsaturated fatty acid extraction experiment by the press way, whose results indicate the yield was much lower than the one done by supercritical CO2 extraction method.The polysaccharide in the defatted Korean pine nut was extracted by water extraction and ultrasonic extraction, whose results indicate that the yield of water extraction is superior to ultrasonic extraction, at the same time determine the best technology parameter of the two kinds of methods respectively. The best technology parameter of water extraction is that temperature 100C, lignin-liquid ratio 1: 10, extraction time is 3.0h, alcohol concentration in precipitation 80%, the best yield is 3.35%; The best technology parameter of ultrasonic extraction is that lignin-liquid ratio 1: 20, extraction time is 1.0h, alcohol concentration in precipitation 80 %.2. Determine the fatty acid content of the Korean pine nut by the chemical methods. The fatty acid that Korean pine nut contained amounts to 70. 26%. Through the analysis of the fatty acid composition, have made clear that the main unsaturated fatty acid forms were polyunsaturated fatty acid, whose main composition are a-linolenic acid and linoleic acid, which are necessary to human body, its content is up to 61.9%. Through the analysis of the primary extraction product - polysaccharide, have determined the content of polysaccharide is 45.76%.3. Regard 22 months aged Wistar rats as experimental objects, carry Korean pine nut unsaturated fatty acid research on delay aging function and regulate blood lipid function. Regard 2 months aged KM mice as experimental objects, research on improvingmemory function and Korean pine nut polysaccharide on immunity regulate function.The experimental study of adjust blood lipid:Korean pine nut unsaturated fatty acid of 4.0 g/kg.d treatment could reduce the level of TG in serum remarkably (P<0.05), lower 31.87% than high fat diet control group (HFD control group), and its also could significantly restrain the increase of rats weight.The experimental study on delay aging:The content of MDA was significantly higher in HFD control group than in 4.0g/kg.d group(P<0.05) and 8.0g/kg.d group(P<0.01), and compared with HFD group, Korean pine nut unsaturated fatty acid could significantly reduce the MDA in serum of rats, of which 4.0g/kg.d group could reduce 12.66%, and 8.0 g/kg.d group could reduce 20.64 %.The oil could also improve the content of SOD, of which 4.0g/kg.d group could improve 10.84%, 8.0 g/kg.d group could improve 14.97%.And the oil could also improve the content of GSH-Px, of which 4.0g/kg.d group could improve 18.25%, 8.0 g/kg.d group could improve 28.47%.And the oil could improve the content of T-AOC too, of which 4.0g/kg.d group could improve 21.18%, 8.0 g/kg.d group could improve 19.38%. And compared with the HFD control group, the differences have statistical meanings.The experimental study to ameliorate memory:The results of experiment to ameliorate memory show that Korean pine nut unsaturated fatty acid could remarkably improve memory of the mice to some extent.The experimental study to adjust immunization:The results of adjust immunization show that Korean pine nut polysaccharide could improve immunity of mice to some extent, especially the weight of spleen in 200.0 mg/kg.d group is hi... |
PDF Full Text Request