Preparation Of Shenning Granues And Effects Of Shenning Granues On Rats With Membranous Glomerulonephritis

Objective To optimize the conditions for the extractions of Radix Astragali, Herba leonuri and Rhizoma Chuanxiong in the preparation of Shenning Granues.To investigate effects of Shenning Granues on nephrotic rats.Methord By orthogonal experimental design, the water decocting techniques were optimized with the dried water decocting rates and contents of starchy trine hydrochloride etc .And the condition of alcohol sedimentation were optimized with the extraction rate of 80% alcohol and contents of starchy trine hydrochloride .Adult male wistar rats were randomly devided into normal control group, pathological pattern group, high-dosage shenning group, middle-dosage group, low-dosage group, Shenfukang group and Prednisone group. Rats with membranous glomerulonephritis were induced by injection of cation bovine serum albumin into tail vein. The rats were treated respectively for 32 days by gavage. Urinary protein was observed once a week after 2 weeks. After 7weeks, plasma albumin, renal histology, parameters of renal function and prostaglandins were examined. In addition TXB2/6-Keto-PGF1. in serum were also examined.Result The optimized decocting condition for Radix Astragalan were to boil the Radix Astragalan for 3 times, each time for 1 hour and add water as much as 8 times. The optimized decocting condition for Herba leonuri and Rhizoma Chuanxiong were to boil Herba leonuri and Rhizoma Chuanxiong for 3 times, each time for 2 hours and add water as much as 12 times, the best condition in the technology of alcohol sedimentation were to condensed the decocting liquid to l:1.5(g:ml) and sediment for 24 hours in 50% alcohol.The urinary protein, serum cretinine level, BUN and TXB2/6-Keto-PGF1. can be reduced in Shenning groups compared with pathological pattern group. Plasma albumin was significantly increased in high-dosage Shenning group.Conclusion The processes of extraction of Radix Astragalan , Herba leonuri and Rhizoma Chuanxiong are feasible .High-dosage and middle-dosage Shenning can significantly decrease urinary protein in rats with membranous glomerulonephritis. High-dosage Shenning can increase the plasma albumin level in rats with membranous glomerulonephritis.