The Spectral Analysis And Study Of Sparfloxacin,Lomefloxacin Hydrochloride And Human Serum Albumin

The dissertation mainly studied the new methods of determination of sparfloxacin, lomefloxacin hydrochloride and human serum albumin. At the same time, it analyzed the spectra of interaction between medicines and eosine B or between protein and some dyes, and the interaction mechanism.On the one hand, two medicines are studied. First, spectrophotometric study on the interaction between eosine B and sparfloxacin. In a Clark-Lubs buffer at pH 2.0, the maximum absorption wavelength of the complex is 530nm with an apparent molar absorptivity 4.98 103L'mor1'cm"1.The peak of the complex has "red shift" of 16nm as compared with that of the reagent. The linear range is 10-50mgL-1, with the detection limit 5.4mg'L-1.The results show that the interaction between eosine B and sparfloxacin mainly is electrostatic attraction. Second, the fluorescence spectral analysis and study on the interaction between eosine B and sparfloxacin. In Clark-Lubs buffer solution at pH 3.5, the excitation and emission wavelength were found to be 308nm and 540nm, respectively. There is a linear relationship in the range of 0.2~4mg'L-1 for sparfloxacin, which is based the fluorescence quenching of eosine B with the addition of sparfloxacin. The detection limit is 0.054 mg'L-1.The method was applied to determine the sparfloxacin capsule. The recovery is 90-109% and the relative standard deviation is 1.2~2.8%. The interaction mechanism was discussed initiatively, which shows that the quenching is static quenching. And the results show that the binding constant of eosine B and sparfloxacin is 7.94 104 and the radio is 1.02. Third, spectrophotometric study on the interaction between eosine B and lomefloxacin hydrochloride. In a Clark-Lubs buffer at pH 1.2, the maximum absorption wavelength of the complex is 535nm with an apparent molar absorptivity 5.28X103L'mor1'cm"1. The peak of the complex has "red shift" of 30nm as compared with that of the reagent. The linear range is 10~40mg'L-1, with the detection limit to be 2.2mgL-1.On the other hand, human serum albumin is studied. First, spectrophotometric study on the interaction between eosine B and human serum albumin. In a Clark-Lubs buffer at pH 1.8, the maximum absorption wavelength of the complex is 528nm with an apparent molar absorptivity 1.19 X 106 Lmol-1 cm-1. The peak of the complex has "red shift" of 14nm as compared with that of the reagent. The linear range is 5~50mg'L-1, with the detection limit to be 0.69mgL-1. The maximum binding number is 200 by using molar ratio method. Second, spectrophotometric determination of human serum albumin byusing indigo carmine. In a Clark-Lubs buffer at pH 1.8, the color of indigo carmine is reduced by the interaction of human serum albumin and indigo carmine. The linear relationship holds between the reduced absorptivity of dye and concentration ranging from 6~34mg'L-1. The proposed method was applied to determine the proteins in human serum with satisfactory results. Third, resonance light scattering method for the determination of human serum albumin with thtrasulponaeted metal phthalocyanine dyes. In a Clark-Lubs buffer at pH 2.0, human serum albumin can bind with thtrasulponaeted metal phthalocyanine dyes to form new products, resulting in the great enhancement of resonance light scattering and new resonance light scattering spectra. The enhanced intensity of resonance light scattering is proportional to the concentration of human serum albumin. It shows that all thtrasulponaeted metal phthalocyanine dyes can determine human serum albumin by resonance light scattering. Four, second-order scattering and anti second-order scattering spectra for the determination of human serum albumin with thtrasulponaeted ferric phthalocyanine. In a Clark-Lubs buffer at pH 2.0, human serum albumin can bind with thtrasulponaeted ferric phthalocyanine to form new products, resulting in the enhancement of second-order scattering and anti second-order scattering spectra. The enhanced intensity of second-order scattering and anti second-order scattering is proportiona...