Studies On Microbial Transformation Of Steroid Intermediate-4AD

The transformation of androstenedione (4AD) was studied to obtain valuable derivatives.A preliminary screening had showed that three strains were efficient for the production of4AD derivatives: Beauveria bassiana HCB-00059, Rhizopus stolonife HCB-00643, andRhizopus stolonife HCB-00644. The metabolites of three strains were isolated and purified.Ten pure compounds were obtained. The structures of six compounds had been identified,the molecular weights of the other four had been known. 1.Four compounds, BB059-1, BB059-2, BB059-3 and BB059-4 were isolated and purifiedfrom Beauveria bassiana HCB-00059 culture. The structures of BB059-2 and BB059-4 wereidentified as 6,11-dihydroxyandrostenedione, 6-hydroxytestosterone. The molecular weightsof the other two compounds , BB059-2 and BB059-4, were 318. 2.Three compounds, RS643-1, RS643-2 and RS643-3, were isolated and purified fromRhizopus stolonife HCB-00643 culture. The structures of RS643-2 and RS643-3 wereidentified as 6-oxo-testosterone and 6,15-dihydroxyandrostenedione. The molecular weightof RS643-1 was 304. 3. Three compounds, RS644-1, RS644-2 and RS644-3, were isolated and purified fromRhizopus stolonife HCB-00644 culture. The structures of RS644-2 and RS644-3 wereidentified as 6-Hydroxyandrostenedione and 14- hydroxyandrostenedione. The molecularweight of RS644-1 was 318. The parameters in the bioconversion process by three strains were optimized. The resultsshowed better progresses.