The Characteristic And Application Of Resonance Rayleigh Scattering Of Selenium Nanoparticle In Liquid System

As a new analytical method, Resonance Rayleigh Scattering has sensitive, simple, rapid, economical feature. The method not only can quantitatively determine trace element or biomolecule, but also can supply scientific and useful information for the research and characterization of nanoparticle or biomolecule. Selenocompounds and the reaction of Au(III) with Selenocompounds were studied with Resonance Rayleigh Scattering method , which has important academic value in fields of seleno medical, food, clinic medicine, health protection nutrition ,etc.The characteristic of liquid Selenium nanoparticle was studied with Resonance Rayleigh Scattering method. In the solution of hydrochloric acid, Selenium(IV) reacted with Vc and formed Selenium(0) that had resonance scattering peak at 470nm. We determined the shape and diameters of Selenium(0) using the technique of TEM and Laser light scattering and we found the shape of Selenium nanoparticle was sphere. The change of the Selenium nanoparticle diameter was consistented with concentration of Selenium( IV). Selenium(IV) with different concentrations had different Resonance Rayleigh Scattering intensities to some extent. The scattering intensity had a good linear relation with the concentration of Selenium(IV) in the range of 0.028-5.640 u g/mL. Many inorganic ions and compounds of sulfur had no influence on Resonance Rayleigh Scattering intensity, but TeO32- Ag+ Au3+ increased the Resonance Rayleigh Scattering intensity.The trace Selenium in Tea and Spirulina Maxima and Astragalus was determined with Resonance Rayleigh Scattering method. The results obtained by the method were consistented with that of DAN (the routine method). Interference experiment and recovery experiment manifested the results were satisfactory.The reaction of amino acids with AuCl3 was studied with Resonance Rayleigh Scattering method . We found selenoamino acids or sulfuramino acids reacted with AuCl3 and formed some substances which had the characteristic of ResonanceRayleigh Scattering. Selenoamino acids reacted with AuCl3 and formed Au(0) and Selenium(0). Au(0) exhibited resonance scattering peak at 590nm and Selenium(0) exhibited resonance scattering peak at 470nm. The reaction speed of Seleno-cystine with AuCl3 was faster than that of Seleno-methionine with AuCl3. The association complex that formed from sulfuramino acids with AuCl3 exhibited maximum resonance scattering peak at 398nm .The scattering intensity increased with increasing of reactant concentration. But other amino acids without the element of S or Se reacted with AuCl3 in acid solution had no obvious phenomenon of Resonance Rayleigh Scattering.