| Amino acids chelated iron (Ⅱ) are usually synthesized in solution,it takes a long time and has liquid wastes. The synthesis of amino acids chelated microelements under microwave radiation in solid state has a rapid speed and a high yielding, the reactant is acetate. In this thesis, amino acids chelated iron (Ⅱ) were synthesized under microwave radiation in solid state, ferrous sulphate was the reactant, proper methods of disacidfying and synthesis technology conditions were studied. According to the reaction conditions, microwave solid state synthesis installation (MSSSI) was preliminarily probed, the biological value of the methionine chelated iron (Ⅱ) was also studied.The proper reaction conditions were decided as follows by single factor experiments: the molar ratio of ligand was 2:1, absorbent was 3.0%, the microwave power was 596 watt, the microwave radiation time (MRT) was from 160s to 260s, the vapour temperature (VT) was from 50.0℃ to 100.0℃, the vapour flow rate was from 0.08 to 0.13g/s, the reactant mass (RM) was from 0.7g to 2.3g. According to the results, the parameters were optimized as follows by current general rotation combination experiments: the VT was 100.0℃, the MRT was 260s, the RM was 1.50g, the best reaction yield was 83.51%. Response surface analysis was also done. The product was characterized by chemical elemental and infrared spectrometry analysis, the constitute of methionine chelated iron (Ⅱ) was established as Fe(Met)2. By simulating the gastrointestinal microenvironment of the rats and detecting the conductivity of the three cheleted iron (Ⅱ) in different pH solution, their stabilities were studied .Results showed that amino acid cheleted iron (Ⅱ) was molecular coordination compounds in liquids and had low molar conductivity, Among them, methionine chelated iron (Ⅱ) and glycine chelated iron (Ⅱ) was stable in pH 2.0~6.0 solution, the histidine cheleted iron (Ⅱ) was stable in pH 2.0~8.0 solution .In order to more effectively remove the byproduct sulphuric acid ,the possibility of disacidfying using vapour A were probed,results showed that vapour A could not only remove the sulphuric acid effectively , but also initiate the reaction slowly and maintain the temperature stability of the reaction system.The ratio of disacidification was about 94.60%.According to the characterization of the reaction, MSSSI was preliminarily probed as continuous upright half cylinder with tray construction,with the synergism of the designed agitation devices and the vapor current , the reactant particles were possibly expanded and suspended . With the synergism of supension and falling stepply of the reactant particles and the "one plate one microwave tube" ,the effective prolong of the reaction time , the homogeneity of microwave radiation and the effectively sulphuric acid transfering were got.In order to probe the biological value of the methionine chelated iron (Ⅱ), anaemic rats were fed with basic diet containing 35mg/kg iron which were ferrous sulphate group,mixed iron group ( ferrous sulphate and methionine chelated iron (Ⅱ) ), methionine chelated iron (Ⅱ) group , results showed that methionine chelated iron (Ⅱ) could notably promote the bady gain of rats, rapidly improve the concentration of hemoglobin, increase the iron content in livers.After 7 days of iron supplements, the growth of the rats of the methionine chelated iron (Ⅱ) group were triple of that of the ferrous sulphate group, the average iron content in livers increased 12.98%.Compare with the 4 days of iron supplements,the serum total iron-binding capacity of the three groups had decreased by 27.86%,25.22% and 39.98% respectively which could basically improve that the biological value of methionine chelated iron (Ⅱ) was the best. |
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