| Chinese herbal drugs are the gems of the five thousands years old civilization of our country. How to develop the advanced analytical methods for the research nature medicine is the challenge task in recent years. In this thesis, nature medicine- Dioscorea Opposita Thunb,Folium Pyrrosiae, Fructus schisandrae was studied by capillary capillary chromatography with UV detection or electrochemical detection and Determination of pharmacokinetic parameter of chlorogetic acid has been carried out by Capillary electrophoresis.This paper consists of five chapters, in the first chapter of this thesis, the history of capillary chromatography was reviewed simply, the application on CE in recent years were introduced；the progress of the determination method for nature medicine was reviewed；the application of CE to nature medicine summarized emphatically.In the chapter II, High performance capillary electrophoresis (HPCE) was applied to the simultaneous separation and determination of allantoin, choline and arginine in Rhizoma Dioscoreae first time in this article. The optimal conditions of electrophoresis such as the pH and concentration of buffer solutions is studied. The three analytes could be well separated within five min by using a phosphate buffer and 24kV as the applied voltage. This method is a rapid, simple and useful technique for identification, separated and determination of allantoin, choline and arginine in Chinese traditional medicine- Rhizoma Dioscoreae. The method is also possible to be applicable to the quality control and directing the growing of Chinese medicine. In the chapter III, Micellar electrokinetic capillary chromatography with UV detection has been employed for the differentiation of North Fructus Schizandrae from South Fructus Schizandrae in the frist time. North Fructus Schizandrae contains a great of Schizandrin A,Schizandrin B and WuWeiZi ester A, but Schizandrin B are not prestent in South Fructus Schizandrae, so those crude drugs can be differentiated by determining their Schizandrin A,Schizandrin B and WuWeiZi ester A content. Effects of several important factors acidity and concentration of running buffer, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. The method was successfully applied to monitor these three compone nts in real samples suches as North Fructus Schizandrae plant fruit and South Fructus Schizandrae plant fruit distilled by water and ethanol.The method also applied to analyzed the simulated urine sample. In the chapter IV, a MEKC method was developed and applied to the simultaneous determination of kaempferol, quercetin and chlorogenic acid in Folium Pyrrosiae Under the optimum condition, the three analytes could be well separated completely within 8 min in a 70 cm capillary (75μm I.D.) of which 60cm was effective length for separation length with a separation voltage of 24kV in a 20 mmol l-1 of the borate buffer with 40 mmol l-1 SDS (PH=8.7). The detection limit of three components was 0.2×10-6μg/ml (chlorogenic acid),0.4×10-6μg/ml (quercetin) and 0.5×10-6μg/ml (kaempferol)separately. Contents of chlorogenic acid, quercetin and kaempferol in the crude drug of Folium Pyrrosiae from different producing area could be easily determined by the proposed method with satisfactory results. In the chapter V,capillary electrophoresis was used for determination of chlorogenic acid in the blood serum, based on which some Pharmacokinetics parameters have been calculated. According to these parameters,the metabolization ofchlorogenic acid was described.In the chapterVI, in order to improve quercetin and chlorogenic acid sensitivity, in this thesis Capillary zone electrophoresis with electrochemical detection (ED) has been employed the separation and determination quercetin and chlorogenic acid in Rhizoma Dioscoreae. Effects of several important factors acidity and concentration of running buffer, separation voltage, injection time and detection potential were investigated to acquire the... |
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