Studies On The Chemical Compositions And Biological Activities Of Serissa Serissoides

In this paper, the chemical compositions of Serissa serissoides and some biological activities, the antibacterial effect and the protective effect against carbon tetrachloride(CCl4)-induced liver injury in mice , have been studied.Five compounds have been isolated from Serissa serissoides by kinds of column chromatography, such as vacuum column chromatography, pressed column chromatography, thin layer chromatography and recrystallization. Three of the five compounds have been identified as Ursolic acid,1.4-dihydroxy-6-methyl-anthraquinone and 1,3,5,7-tetramethoxy-anthraquinone respectively by IR, 1HNMR, 13CNMR and MS analysis. The chemical structures of other two compounds are still being studied. Based on GC-MS analysis, one mixture of sterols has been identified as cholestrol, ergosta-7,22-dien-3-ol, 4-methylcholestrol, stigmasterol and P-sitosterol. Among them, stigmasterol and P-sitosterol are the main component, the percentages of which are 35.564 % and 52.694 % respectively.The volatile constituents of the essential oil of Serissa serissoides extracted by steam distillation have been studied by GC-MS. A total of 43 components have been identified representing the 88.87 % of the oil.The antibacterial effect of some samples of Serissa serissoides on six strains of bacteria such as E. coli has been studied. The minimal inhibitory concentration (MIC) of different samples to various bacteria has been determined by way of double-dilute method. Almost all the samples showed weak antibacterial effect in the range of the experimental concentration.The protective effect of the Serissa serissoides solution on carbon tetrachloride(CCl4)-induced acute hepatic injury in mice has been also studied by the measurement of ALT, AST and MDA. The results showed that the Serissa serissoides solution caused the reduction of the elevated enzyme levels of ALT and AST significantly, but didn't reduce the level of MDA. It suggested that the Serissa serissoides solution had a significant efficiency on a CCl4-induced acute hepatic injury, but the protective effect might be not related to anti-free radical.