Isolation, Purification And Chemical Stucture Of Polysaccharide From Pleurotus Ferulae Lanzi

Isolation, Purification and Chemical Stucture ofPolysaccharide from Pleurotus ferulae LanziPostgraduate: li Jun; Director: Lu Zuo-zhou (Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070)In this thesis, the isolation, purification and the structural characteristics of polysaccharide PW2 from the fruit body of Pleurotus ferulae Lanzi were studied. Mushroom powder was obtained by dry, grind after smashing from the fruit body, and was withdrawn by hot water and were precipitated by alcohol to get the protein-bond polysaccharide(PW). And then PW were taken off protein with the method of Sevag, used the distilled water dialysis to remove the ion. After DEAE-Cellulose ion change column chromatography, there were three polysaccharides PW1, PW2 and PW3, the color was thin and yellow respectively, white, thin and yellow. Finally the PW2 was regarded as the sample proceeding the construction measurement research.Passing UV spectrum, PW2 does not contain the protein and nucleic acid, and was proved to be pure through PC, gel filtration and electrophoresis. Its molecular weight were estimated to be 3.18 104 by HPLC. PW2 are completely hydrolyzed catalyzed by acid, to have the bond of indicant broken entirely and get the contents of sugar, measured by HPLC. PW2 are mainly made up of D-Glc and D-Gal by 1. 77:1 in molar. Analyzed by Infrared Ray(IR) and Nuclear Magnetic Resonance(NMR), The structure of PW2 is chiefly linked by a -glycoside bonds and a few glycoside bonds, while the backbone of PW2 is mainly linked by 1-6 glycoside bonds and a few 1-4 linkage.