Studies On Electrochemical Probe Of Protein And Its Analytical Application

Protein is an important substance in human body and it can easily bind with the inner and exo substances to influence the healthy of human. The qualitative and quantitative analysis of protein is also an important factor in biochemical and clinical assays. Electroanalytical chemistry is one of the most interesting research techniques that can be used to investigate the structures and mechanisms of biomolecules. This thesis combines with the analytical determination of protein with electroanalytical chemistry and focuses on the study of the electrochemical probes of protein and analytical application. The paper can be summarized as follows:1. The electrochemical probes of protein are carefully studied. The interaction of small molecules such as acid chrome blue K, chromotrope 2B, chromotrope 2R, thorin, chromazurol S were selected and used as electrochemical probes for the determination of protein with different electrochemical method such as linear sweep voltammetry, cyclic voltammetry and UV-Vis spectrophotometric method. The small organic molecules are electrochemical active and can be reduced on the mercury electrode. On the addition of protein, the reduction peak current decreases because of interaction. The decrease of reductive peak current is proportional to the protein concentration. The conditions of binding reaction and the electrochemical determination were optimized. The influences of coexisting substances such as metal ions and amino acids were tested. Under the optimal conditions the working curves for different proteins were established and the proposed methods were further applied to the detection of the content of albumin in healthy human serum samples. The...