Study On Isolating And Primary Growing Conditions Of Carotenoids Producing Bacteria Strain PBH(Kurthia Sp.) And Its Producing Pigments

Carotenoids belong to the tetraterpenes family and are represented by more than 600 known natural sructural variants. They are divided in two categories, carotenes containing only carbon and hydrogen atoms and oxocarotenoids (xanthophylls) which carry at least one oxygen atom. Carotenoids are synthesized in plants, fungi, bacteria and algae. In recent years, medicinal studies indicate that carotenoids play an important role in human health", such as quenching free-radical, improving human immunity, preventing ageing, cardiovascular disease, some types of cancer and some chronic disorders, inhibiting cataract and so on. Due to the protective effects of the carotenoids, they have been abroadly applied in food, medicine, health care, animal feedingstuff, chemical industry and cosmetic industry.In this report, dozens of bacterium strains that producing carotenoids were isolated from the samples of the soil polluted by oil, faded flowers, medium of plant tissue culture and medium of Luria-Bertani gelose flat contaminated by bacteria. One bacterium strain PBH that produces yellowcarotenoids that isolated from the contaminated medium of LB gelose flat was identified and classified as Kurthia sp through morphologic and biochemical experiments.The producing pigments of PBH was extracted and have been identified as some kind of lutein after examined by spectrophotometer, thin-layer chromatography and high-performance liquid chromatography.PBH's physiological conditions were investigated, too. We found PBH strictly aerobic, growing well in liquid medium pH of 7. 2~7.4, on temperature of 28癈 under light. In the laterly additives experiments, PBH's cell biomass and carotenoids yield and content could reach 65.57 mg wet cell per mL medium, 9.896 ug per ml medium and 150.9 Hg per gram wet cell respectively, when growing in the medium containing glucose(40g/L) as carbon source, adding tomato juice(3. 6mL/L), gingili oil(1.6mL/L), Na,C03(6g/L) and phosphate buffer, at 28C with illumination for 120h.