Separation And Detection Of DNA And IgG Based On The Nanochannels

As an important part of Nanoscience, nanochannels technique has become a new growth point. Nanochannel is the pores or tubles with diameter from 0.1 to 100 nanometers. It is exhibiting the great potential advantage and promising future due to the size effect and chemical physical characteristics. Nanochannel technique involves many disciplines such as molecule biology, bio-chemistry, electronics, material science, informatics and so on. Little paper about nanochnnel has been reported inboard.Separation and detection of analytes are two important aspects of nanochannel techniques. Although some papers on the single channel were done, many new works on the multinanotuble array for separation and detection of biomolecule are waiting us to do. These work include how to obtain the nanochnnels with the selectivity groups through which molecule can be choosed to pass, how to enhance the speed and flux of separation and develop new detection method based on nanochannels and so on. Hereby, main work in the present thesis are shown as follows:1. Au nanotubules (channels) can be electrolessly plated within the pores of polycarbonate microporous filtration membranes. Au nanotubules with large numbers of pores can be modified with groups and the diameter can be controlled at will. The transportation behaviour was studied when the bio-molecules pass through the Au nanotubules. Based on the research of behaviour of nanochannels, we developed the techniques of immunodetection and DNA analysis and separation.2. With the existence of an electric field, a response current can be recorded when sheet anti-human IgG (SAHIgG) moved through the channels. After HIgG was added to the feed cell, formation of the SAHIgG- HIgG complex due to immunoreaction in the solution made intensive baffle event and the current decreased. HIgG can be determined by the current difference.3. Anavidin-coated nanoparticles and nanochannels were modified with biotinylated ssDNA2 and ssDNAl, respectively. A sandwich structure will be formed by sequences of DNA1, DNA2 and target DNA3 if these DNA segments are complementary. The impendance of the film with nanochannels increased due to the blocking event from nanoparticles. The higherconcentration of DNA made more impendance. On the other hand, single base mismatched DNA4 a nd random DNA5 c annot change the impendance of the film. The TDNA3 can be detected by monitoring the AC impendance signals.4. In our work, nanochnnels was originally labeled with DNA chains. As the result, target DNA can selectively pass through the film under an electric field. Some factors relate to DNA transportation was studied such as temperature, voltage, the diameter of channel and so on. In our work, target DNA moved through nanochnnels with notable higher velocity than others. In other words, the TDNA can be choosed to pass through the nanochannels. Based on this principle, target DNA is separated from several types of DNA at will.In summary, we developed some new approach for bioanalysis and biodetection by nanochannels in combination with photochemistry and electrochemistry. These works will greatly benefit the research on analysis and purification of bio-molecules and develop nanochannel techniques. We expect this method to be widely and deeply studied and became twinkling point of nanotechnology.