The Effects Of Pressure On Synthesis Of Hydrocortisone By Absidia Coeralea

The dissertation discusses effects of high pressure on solubility of R.S.A(17α -hydroxypregna - 4 -ene-3,20-dione-21 -acetate) and viability of spore of Absidia coerulea.Mainly conducted research on effects of pressurize fermentation on biosynthesis of Absidia coerulea ,include the relationship between different gas, treatment methods and hydrocortisone conversion ratio.Confirm the relativity of dehydrogenase activity and hydrocortisone conversion ratio. 1. Reseached the effects of high pressure on solubility of R.S.A,found a certain high pressure,treatment time and temperature could efficiently enhance the solubility of R.S.A,accelerated the conversion reaction.The solubility was no difference in using different gases.Ensured solubility equation of R.S.A, lnC = -1.1459 + 0.12671nP-37.323/T,C denoted solubility of R.S.A;P denoted pressure, unit was MPa;T denoted absolute temperature,unit was K. 2. Discussed when used different gases as medium,the effects of pressure, treatment time and rate of comprion and decompression on viability ratio,and analyse the cause. Under proper value of pressure, treatment time and rate of comprion and decompression, Absidia coerulea could keep a certainviability(>11%)3. Research the effects of pressurize fermatation on biosynthesis of Absidia coerulea,found pressure value,treatment time,during different phase process pressurize fermentation made the obvious effects to biosynthesis of Absidia coerulea .Experiment showed none-oxygen gas(such as nitrogen),excessive high pressure value(>1MPa),excessive treatment time(>8h) could make severe effects on conversion reaction.Established proper pressurize fermentation condition:during the conversion reaction phase,after 36h conversion reaction at 0.1MPa,then used high pure air as medium and under 0.5MPa processed pressurize fermentation for 6h(adding oxygen-vector into culture medium),followed 6h conversion at 0.1MPa,the conversion ratio of hydrocortisone could increased 32.4%.4. Approved the relativity between conversion ratio of hydrocortisone and dehydrogenase activity,explained ehydrogenase mainly use the intensity of transfer hydrogen through respiration chain to effect hydroxylation.5. Employed the SEM to observe morphology of Abasidia coerulea ,discovered the difference when used different gases.Hypha body was very dense under 0.1 MPa and there was almost no space between hypha body.Compared thefermentation under 0.1MPa,after pressurize fermentation the space of hypha body was larger. When used nitrogen as medium,the space of hypha body was largest,related to the restrain effect of Abasidia coerulea growth. When used high pure air as medium,compared the result of using nitrogen,although there was a little space between hypha body,more dense of hypha body and more bloom of growth.